Gene Delivery System Using Thermo-responsive Polymers
| Project/Area Number |
11650913
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
高分子合成
|
|---|
| Research Institution | Tokyo Woman's Medical University |
|---|
Principal Investigator |
YOKOYAMA Masayuki School of Medicine, Tokyo Woman's Medical University, Asistant Professor, 医学部, 助教授 (20220577)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Thermo-response / Synthetic Polymer / Gene / DNA / Delivery / Synthetic Vector / Gene expression / Complex / コンブレックス / 高分子 / ベクター / ドラッグデリバリーシステム / ドラックデリバリーシステム |
|---|
| Research Abstract |
A thermo-responsive copolymer, poly(N-isopropylacrylamjde (IPAAm)-co-2-(dimethylamino)ethyl methacrylate (DMAEMA)-co-butylmethacrylate (BMA)) was synthesized, and its in vitro gene transfection efficiency at different incubation temperatures was evaluated. A copolymer containing 8 mol% of DMAEMA and 11 mol% of BMA (P(IP-8DA-11BM)) had a lower critical solution temperature (LCST) at 21℃, therefore, the copolymer was insoluble above 21℃ and soluble below 21℃. This copolymer was complexed with plasmid DNA. Transfection efficiency of polymer-plasmid complexes was evaluated in COS-1 cells using pCMV-βgal plasmid, encoding for β-galactosidase as a reporter gene. Transfection efficiency of PDMAEMA homopolymer incubated at 37℃ for 48h was higher than that incubated at 20℃ for 3h and 37℃ for 45h. In contrast, transfection efficiency of P(IP-8DA-11BM) incubated at 20℃ for 3h and 37℃ for 45h was much higher than that incubated at 37℃ for 48h. This gene expression enhancement was increased to 8.6-fold by optimizing incubation period of the complex with cells. Such increased transfection efficiency by lowering temperature is considered to be due to appropriate formation/dissociation control of P(IP-8DA-11BM)-DNA complexes.
|
Report
(4 results)
Research Products
(15 results)
