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Molecular and functional analyses of the induced mutable gene slg in rice.

Research Project

Project/Area Number 11660003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TANISAKA Takatoshi  Kyoto University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (80026591)

Co-Investigator(Kenkyū-buntansha) HORIBATA Akira  Kinki University, Faculty of Biology Oriented Science and Technology Research Associate, 生物理工学部, 助手 (70258060)
NAKAZAKI Tetsuya  Kyoto University, Graduate School of Agriculture, Research Associate, 農学研究科, 助手 (60217693)
OKUMOTO Yutaka  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (90152438)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsrice / mutable gene / YAC / transposon / slender glume gene / 易変性遺伝子
Research Abstract

A mutable slender glume gene slg was induced by gamma-ray irradiation to seeds of the rice variety Gimbozu. Occasionally reverting to its wild-type, slg is assumed to involve an active transposable element in it. To identify the molecular characteristics of this element, we attempted to clone slg with a positional cloning method. slg has already been mapped on the overlapping region of two YAC clones, Y1774 and Y3356. We first selected 209 candidate clones from the cDNA clone library of the rice variety Nipponbare using a YAC clone Y1774 as a probe, and then partially sequenced 54 clones. Based on the sequence data, 40 clones were found to be independent of each other. Using these 40 clones as probes, we finally selected out cDNA clones that hybridized with both YAC clones. But many of them were hybridized not only with both YAC clones but also with multiple regions of genomic DNA, indicating the occurrence of non-specific hybridization. Thus, it was difficult to identify the clones other than No.27 that was really located on both YAC clones. Southern analyses were also conducted to find out polymorphic bands among the original variety, mutant line (slg) and revertant (wild-type). Consequently, polymorphic bands were observed when using clone No.120, suggesting that this clone is tightly linked with slg locus. The effect of slg on the genomic methylation was also investigated because there was a possibility that the mutability of slg was caused by DNA methylation. The mutant line was significantly lower than the original variety in the relative amount of methylcytosine to total cytosine on MspI site. This indicates that slg may have a decreasing effect on the genomic methylation, resulting in the mutability of slg.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Teraishi,M.,H.Hirochika,Y.Okumoto,A.Horibata,H.Yamagata and T.Tanisaka: "Identification of YAC clones containing the mutable slender glume locus slg in rice (Oryza sativa L.)"Genome. 44. 1-6 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakazaki,T.,N.Ihara,Y.Fukuta,and H.Ikehashi: "Abundant polymorphism in the flanking region of two loci for basic PR-1 proteins as markers for indica-japonica differentiation in rice (Oryza sativa L.)"Breeding Science. 50. 173-181 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 堀端章,山縣弘忠: "イネ細粒遺伝子slgの復帰突然変異性およびそれが突然変異誘発因子の活性に及ぼす影響"育種学研究. 2. 125-131 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teraishi,M.,Y.Okumoto,H.Hirochika,A.Horibata,Y.Yamagata and T.Tanisaka: "Identification of a mutable slender glume gene in rice (Oryza sativa L.)"Molecular and General Genetics. 261. 487-494 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Pan,Q.H.,L.Wang and T.Tanisaka: "A new blast resistance gene identified in the Indian native rice cultivar Aus 373 through allelism and linkage tests"Plant Pathology. 48. 288-293 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teraishi, M., H.Hirochika, Y.Okumoto, A.Horibata, H.Yamagata and T.Tanisaka: "Identification of YAC clones containing the mutable slender glume locus slg in rice (Oryza sativa L.)"Genome. 44. 1-6 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakazaki, T., N.Ihara, Y.Fukuta and H.Ikehashi: "Abundant polymorphism in the flanking region of two loci for basic PR-1 proteins as markers for indica-japonica differentiation in rice (Oryza sativa L.)"Breeding Science. 50. 173-181 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Horibata, A.and H.yamagata: "Reversion mutability of a slender-glume gene slg and its influence on the activity of a mutagenic factors in rice"Breeding Research. 2. 125-132 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teraishi, M, Y.Okumoto, H.Hirochika, A.Horibata, Y.Yamagata and T.Tanisaka: "Idnetification of a mutable slender glume gene in rice (Oryza sativa L.)"Mol.Gen.Gen. 261. 487-494 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Pan, Q.H., L.Wang and T.Tanisaka: "A new blast resistance gene identified in the Indian native rice cultivar Aus 373 through allelism and linkage tests"Plant Pathology. 48. 288-293 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teraishi,M.H.Hirochika,Y.Okumoto,A.Horibata,H.Yamagata,and T.Tanisaka: "Identification of YAC clones containing the mutable slender glume locus slg in rice (Oryza sativa L.)"Genome. 44. 1-6 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakazaki,N.Ihara,Y.Fukuta,and H.Ikehashi: "Abundant polymorphism in the flanking region of two loci for baic PR-1 proteins as markers for indica-japonica differetioation in rice (Oryza sativa L.)"Breeding Science. 50. 173-181 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 堀端章,山縣弘忠: "イネ細粒遺伝子slgの復帰突然変異性およびそれが突然変異誘発因子の活性に及ぼす影響"育種学研究. 2. 125-131 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 堀端章: "易変細粒遺伝子slgの復帰突然変異の組織培養による制御"育種学研究. 1(別冊2). 246 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Teraishi: "Identification of a mutable slende glume gene in rice"Mol.Gen.Genet.. 261. 487-494 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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