| Project/Area Number |
11660004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Okayama University |
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Principal Investigator |
TAKEDA Kazuyosi Res.Inst.Biores. Okayama University, Professor, 資源生物科学研究所, 教授 (90003516)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Takashi Biotec.Res.Sapporo, Okayama University, Chief Res., 研究員
SATO Kazuhiro Res.Inst.Biores, Okayama University, Asso.Prof., 資源生物科学研究所, 助教授 (60215770)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | barley / genetic resources / varietal variation / beta-amylase / enzymatic polymorphysm |
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| Research Abstract |
The beta-amylase of barley is one of the important enzyme in the mashing process during beer production.
The termostability of beta-amylase was surveyed in about 6,700 lines of barley genetic resouces inorder to find new and useful mutant gene and to realize the geographical distribution of the beta-amylase genes. Most of the lines were categorized into the types A, B and C.The proportion of the types A, B and C was 52%, 22% and 26%, respectively. The thermostability types in southwest Asia consisted of Types A, B and C.Most of the East Asian lines were Type A.More than 95% of the Ethiopian barley was type C.
Besides types A, B and C, we found new thermostability types including a beta-amylase-less mutant and a highly thermostable mutant.
In addition to thermostability the beta-amylase isozyme type was surveyed using means of Isoelectric Focusins (IEF). Thermostability type A and C lines were grouped into IEF-I type, and lines of type B consisted of the IEF-I and II types in the preliminary test. The IEF isozymes of beta-amylase were then surveyed in about 1,550 lines of the thermostability type B lines. The IEF type-II was 85% and the rest was the IEF type I.The IFF type-I is distributed mainly in the northern Europe.
A QTL analysis using the Steptoe/Morex (type B II/ B I) doubled haploid lines revealed that a QTL existed around the Bmyl locus.
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