| Project/Area Number |
11660021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HANDA Takashi University of Tsukuba, Institute of Agriculture and Forestry, Assistant Professor, 農林学系, 講師 (00192708)
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| Co-Investigator(Kenkyū-buntansha) |
KURASHIGE Yuji Niigata Botanical Garden, Senior Researcher, 研究官
YUKAWA Tomohisa Tsukuba Botanical Garden, National Science Museum, Curator, 筑波研究資料センター, 研究官 (50280524)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Rhododendron / Menziesia / Molecular Phytogeny / Interpeneric Hybrid / Amplified Fragment Length Polymorphisms / ITS / ツツジ属 / 遠縁交雑 / matK |
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| Research Abstract |
To develop new cultivar groups in the genus Rhododendron, sequence analysis and AFLP analysis were performed. As a result of matK region of CPDNA and ITS region of nrDNA in Rhododendron and its close related species, taxonomic position of some species should be reconstituted in the genus Rhododendron. All five species of the genus Menziesia were included in the clade Rhododendoron, which indicates Menziesia might be a submenus of Rhododendron. AFLP analysis could reveal the genetic diversity of Menziesia.
Some combinations for cross-hybridization between M multittora and several species of Rhododendron produced seedlings. These seedlings were analyzed for AFLP analysis to confirm the hybridity. Microscopic observation of elongation of pollen tube and embryo development were performed to clarify the reason of difficulty in some combinations.
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