Studies on the mechanism for priming effect on seed germination
| Project/Area Number |
11660022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Saitama University |
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Principal Investigator |
MOTOHASHI Yukio Saitama University Science Prefessor, 理学部, 教授 (70015113)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | priming / seed germination / endo-β-mannanase / endosperm |
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| Research Abstract |
The mechanism for the improvement of germination brought about by priming treatment was studied.
We firstly showed that endo-β-mannanase, which is responsible for breakdown of tomato endosperm cell walls, developed exclusively in the endosperm region (micropylar region) just adjacent to the radicle tip before radicle protrusion (germination) in unprimed tomato seeds. The hydrolysis of mannans, which is the major constituent of cell walls of tomato endosperm, by the enzyme activity probably brings about the weakening of mechanical restraint against radicle growth (germination) at the micropylar region of the endosperm surrounding the radicle tip. Hence a working hypothesis was provided that the breakdown of cell walls in micropylar portion of the endosperm also occurs during priming treatment. It was clearly shown that endo-β-mannanase actually developed specifically in the micorpylar region of the endosperm during priming treatment. We prepared the antibodies against this enzyme, and by immunoblotting using the antibodies the form of the enzyme detected in primed seeds was shown to be the same as that developing during germination of unprimed seeds. Electron-microscopic observations indicated that the cell walls of the micropylar region appeared eroded to some degree in primed seeds.
These results strongly support the hypothesis that endo-β-mannanase development in the micorpylar region of the endosperm during priming is involved in the improved germination of primed seeds.
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Report
(3 results)
Research Products
(7 results)
