| Project/Area Number |
11660025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Gifu University |
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Principal Investigator |
MATSUMOTO Shogo Gifu Univ., Associate Prof., 教育学部, 助教授 (90241489)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Apple / Self-incompatibility / Malus / S-locus / S-allele / PCR-RFLP / Rosaceae / Cross-incompatibility / S遺伝子型 / バラ科植物 / S-RNase / PCR |
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| Research Abstract |
( 1 ) The S-locus genes ( Sd-, Sh-, Se- and Si-RNase ) in the pistil were cloned and sequenced from the apple (Malus x domestica Borkh. ) cultivars. We developed an S-allele-specific polymerase chain reaction ( PCR )-restrictioon fragment length polymorphism ( RFLP ) analysis method for identification of the S-alleles. Using that method, we analyzed S-genotype of 136 apple cultivars and selections.
( 2 ) The St- and Sg'-RNase CDNA were cloned from Malus trcmsitoria. S-allele genotypes of nine Malus species were identified by S-allele-specific PCR-RFLP analyzes.
( 3 ) New S-RNases, Sg and Sh-RNase, and Sf-RNase show a strong similarity ( 93-94 % ) to each other at the deduced amino acid level. There are only two to six amino-acid differences among HV regions responsible for the S-allele specificity of Sf, Sg and Sh.
( 4 ) Sg'-RNase differed by one amino acid from that of Sg-RNase. A difference of one amino acid within HV regions produced by natural mutation was not enough to generate a new S-allele. However, the model ofMaton et al. ( 1999 ) on establishing novel S-alleles was supported by one amino acid difference since S_g-allele could recognize both S_g- and S_g- alleles.
( 5 ) Based on the phylogenetic analysis of S-RNases, it was speculated that the polymorphic S-RNases in apple were generated before divergence into the genera Malus and Pyrus.
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