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molecular analysis of mechanism of fruit ripening and softening in pears

Research Project

Project/Area Number 11660032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KUBO Yasutaka  Okayama University Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80167387)

Co-Investigator(Kenkyū-buntansha) NAKANO Ryohei  Okayama University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (70294444)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPear / Fruit softening / Fruit Ripening / Ethylene / Expansin / Polygalacturonase / Gene expression / 成熟
Research Abstract

In order to establish physiological base of postharvest technology for extended shelf life with better quality, we investigated the mechanism of fruit ripening and softening at molecular level. We isolated ethylene biosynthetic gene (four isogenes for ACC synthase and two isogenes for ACC oxidase) and genes encoding cell wall modifying (two isogenes for polygalacturonase, two isogenes for cellulase and eight isogenes for expansin). The ripening ethylene production declined with decrease of transcripts for ACC synthase and ACC oxidase by 1-MCP, a strong inhibitor of ethylene action when it was applied to fruit after onset of climacteric ethylene. The results indicate that ripening ethylene biosynthesis in pear fruit is regulated in positive feedback manner. In addition of suppression of ethylene biosynthesis, 1-MCP application during ripening stage significantly delayed the subsequent fruit softening, resulting in extended shelf life more than twice of non-treated fruit. In the 1-MCP treated fruit the expression of three isogenes for expansin and two for polygalacturonase was suppressed while other members of expansin and two cellulase genes were not affected. These results suggest that expression of some expansin and polygalacturonase genes depends on ethylene and plays an important role in fruit softening. We concluded that application of 1-MCP and metabolic engineering of ethylene biosynthetic gene such as ACC synthase and ACC oxidase promise advanced postharvest technology in transportation and marketing in pear fruit.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 日和佐京子,中野龍平,久保康隆,稲葉昭次.: "セイヨウナシ果実のエクスパンシン遺伝子のクローニングと発現解析"園芸学会雑誌別冊. 68-2. 443 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 衣笠雄一,日和佐京子,中野龍平,久保康隆,稲葉昭次.: "セイヨウナシ果実におけるエチレン生合成関連遺伝子の全鎖長cDNAの単離とその発現解析"園芸学会雑誌別冊. 69-1. 411 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 日和佐京子,天野聡美,橋本晶子,中野龍平,久保康隆,稲葉昭次: "セイヨウナシ果実の追熟に伴うPGおよびEGasa遺伝子の全鎖長cDNAの単離とその発現解析"園芸学会雑誌別冊. 70-1(発表予定). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiwasa, K., R.Nakano, Y.Kubo and A.Inaba.: "Molecular cloning of genes encoding expansin and thier expression in pear fruit"J.Japan.Soc.Hot.Sci.. 68-2. 443 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kinugasa, Y., K.Hiwasa, R.Nakano, Y.Kubo and A.Inaba.: "Molecular cloning of full length cDNAs for ethylene biosynthetic genes and thier characterization in pear fruit"J.Japan.Soc.Hot.Sci.. 69-1. 411 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiwasa, K., S.Amano, A.Hashimoto, R.Nakano, Y.Kubo and A.Inaba.: "Molecular cloning of full length cDNAs encoding PG and Egase and their expression in relation to fruit ripening in pear"J.Japan.Soc.Hot.Sci.. 70-1 (In press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 衣笠雄一,日和佐京子,中野龍平,久保康隆,稲葉昭次.: "セイヨウナシ果実におけるエチレン生合成関連遺伝子の全鎖長cDNAの単離とその発現解析"園芸学会雑誌別冊. 69-1. 411 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 日和佐京子,天野聡美,橋本晶子,中野龍平,久保康隆,稲葉昭次: "セイヨウナシ果実の追熟に伴うPGおよびEGasa遺伝子の全鎖長cDNAの単離とその発現解析"園芸学会雑誌別冊. 70-1(発表予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 日和佐京子,中野龍平,久保康隆,稲葉昭次: "セイヨウナシ果実のエクスパンシン遺伝子のクローニングと発現解析"園芸学会雑誌68別2. 68. 443-443 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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