Genetic linkage analysis of ecological traits and molecular markers in Lilium
| Project/Area Number |
11660033
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Kyushu University |
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Principal Investigator |
HIRAMATSU Michikazu Fac. Of Agriculture, Graduate School, Kyushu University, Assistant Prof., 大学院・農学研究院, 助手 (30264104)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Lily / Lilium longiflorum / Lilium formosanum / ecological trait / genetic analysis / genetic linkage map |
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| Research Abstract |
The aim of the present research is to reveal evolutionary and genetic backgrounds of ecological traits that significantly differentiate closely- related, and -distributed species, Lilium longiflorum and L. formosanum.
Population genetic analysis based on allozyme variations suggests that L. formosanum is a derivative from southern peripheral populations of L. longiflorum around the late Pleistcene. The `early flowering` trait characterized by virgin flowering coming within a year from sowing in L. formosanum seemed to be reflected in the significantly larger annual net production in a plant body above the ground than that of L. longiflorum, even in the case that the annual net production in an underground part was comparable between the species.
All F_1 progenies generated from crosses between L. formosanum and L. longiflorum flowered within a year from sowing. Self-compatibility and summer flowering traits of L. formosanum appeared almost dominantly in F_1 progenies. These results indicate that accumulated dominant mutations for such ecological traits in an ancestral population promote speciation of L. formosanum from L. longiflorum.
Progenies in F_2 were segregated into ones with or without the `early-flowering` trait originated from L. formosanum. The `early-flowering` was appeared in individuals with the larger net production. This indicates that genes controlling net production closely concern the `early flowering`.
Construction of genetic linkage map with randomly amplified polymorphic DNA (RAPD) markers was quite difficult, since stably-resolved RAPD markers were not obtained sufficiently. Thus, mapping of molecular markers and Quantitative trait loci (QTL) should be conducted using such alternative stably-resolved DNA markers as amplified fragment length polymorphism (AFLP).
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Report
(4 results)
Research Products
(6 results)
