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Distribution, usefulness, and host specificity of grass endophytes

Research Project

Project/Area Number 11660055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionResearch Institute of Agricultural Resources, Ishikawa Agricultural College

Principal Investigator

KOGA Hioronori  Research Institute of Agricultural Resources, Ishikawa Agricultural College, Professor, 附属農業資源研究所, 教授 (60290079)

Co-Investigator(Kenkyū-buntansha) NAKAYACHI Osamu  Research Institute of Agricultural Resources, Ishikawa Agricultural College, Assistant, 附属農業資源研究所, 助手 (40227963)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsgrass endophytes / symbiosis / Neotyphodium / Epichloe / Ephelis / host specificity / immuno-electronmicroscopy / incompatibility / エネルギーフィルター電子顕微鏡 / EF-TEM / 透過性 / 元素分析 / レクチン / トールフェスク / ペレニアルライグラス
Research Abstract

Grass endophytes have been paid attention to utilize in agriculture, because the endophyte-infected plants enhance the resistance to the certain insects and plant pathogens. In order to utilize the endophytes in agriculture, various kinds of endophytes should be collected in Japan and from over seas, thereafter collected endophytes should be isolated, identified and examined the usefulness such as enhancement of resistance to insects and plant pathogens. Especially the specificity between plant and endophyte is a bottleneck when the other species of plants are infected by artificial inoculation with fungal endophytes. Therefore, the researches have been forcused on the specificity of compatibility and incompatibility by using electron microscopy.
The results are as follows, 1) Not only symptomless (Neotyphodium) endophytes but also stigma-formed **e (Epichloe) endophytes were collected and identified, also fungal endophytes were collected from C4 plants as well as C3 plants, 2) It has been clarified in both C3 and C4 plants that endophyte-infected plants enhanced the resistance to the certain insects. 3) A detection method for the fungal endophyte by immuno electron microscopy was established. 4) It was suggested that the contents of intercellular matrix where the specificity was expressed were derived from host plant rather than endophyte hyphae by using immuno electron microscopy. 5) The contents of intercellular matrix were also compared between compatible and incompatible combinations by using energy filter electron microscopy. As the results, there were large differences between both combinations on the detection of O and P and so on. The mechanisms of host specificity were discussed from these results.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 古賀博則, 中谷内修: "トールフェスク及びペレニアルライグラス組織内のNeotyphodiumエンドファイト菌糸の免疫電子顕微鏡法による検出"日本植物病理学会報. 66. 179 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則: "エンドファイト(植物内部共生菌)のコロイド金抗体法を用いたSEMによる検出"医学生物学電子顕微鏡技術学会第8回医学生物学電顕シンポジウムプログラム予稿集. 17 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Ultrastructure of pathogenic and endophytic fungi/plant interfaces"Proceedings of 7th Symposium of mycological Society Japan. 45-48 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則, 秋山典昭, 中谷内修: "Post-embedding法による免疫電顕のNeotyphodiumエンドファイト(植物内生菌)への応用"医学生物学電子顕微鏡技術学会第17回学術講演会および総会プログラム・予稿集. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則, 月星隆雄, 植松勉, 島貫忠幸, 但見明俊: "わが国におけるチューイングフェスクおよびヤマカモジグサがまの穂病の発生"草地試研報. 57. 27-34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Fungal endophytes of grasses in Japan : benifits to the host and problems. Mycotoxin contamination : helth risk and prevention project"Mycotoxins Supplement. 99. 131-137 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H., Tsukibosh, T., Nakayachi, O.: "Occurrence of black chokes of Eragrostis ferruginea (Thunb.) P.Beauv. and Pennisetum alopecuro ides (L.) Spreng, caused by Ephelis sp."Bull.RIAR.Ishikawa Agr.Coll.. 6. 45-52 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "食植生昆虫類から身を守る植物の共生戦略"個体群生態学会報. 56. 47-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 上垣隆一, 古賀博則, 神田健一, 島貫忠幸: "エフェリス・エンドファイト感染スズメノヒエのコガネイナゴに対する摂食忌避作用"草地学会誌. 46. 74-76 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "エンドファイトの耐虫性付与と宿主特異性機構"IGEシリーズ26.微生物の共生戦略の分子機構と多様性. 145-157 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "草地とエンドファイト"(日本草地学会)世界の草原と私たち(塩見正衛編). 34-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "エンドファイト(植物内部共生菌)のコロイド金抗体法を用いたSEM検出"医学生物学電子顕微鏡技術学会誌. 16(1). 43-45 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則, 秋山典昭, 月星隆雄, 中谷内修: "Neotyphodiumエンドファイト菌糸のpost-embedding法によるコロイド免疫電顕"医学生物学電子顕微鏡技術学会誌. 16(2). 41 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "エンドファイト研究の現状と展望"植物防疫. 55. 475-478 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則, 栗原孝行, Christensen, M.J., Bennett, R.J., 中谷内修: "Neotyphodiumエンドファイトと寄主植物との接触場面のエネルギーフィルター電子顕微鏡による解析"医学生物学電子顕微鏡技術学会誌. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀 博則: "エンドファイトのはなし"理研グリーン. 90 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H., Tsukiboshi, T., Uematsu, T., Shimanuki, T. and Tajimi, A.: "Occurrence of choke discases of cheming fescue and slender false brome grass in Japan"Bull. Natl Grassl. Res. Inst.. 57. 27-34 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Fungal endophytes of grasses in Japan : benifits to the host and problems"Mycotoxins Supplement '99. 131-137 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H., Tsukiboshi, T. and Nakayachi, O.: "Occurrence of black chokes of Eragrostis ferruginea (Thunb.) P. Beauv. and Pennisetum alopecuroides (L.) Spreng. caused by Ephelis sp. in Japan"Bull. RIAR. Ishikawa Agr. Coll., 6. 45-52 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Utilization of endophyte-intected plants for increased insect resistance."Annuals of Population Ecology. 56. 47-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uegaki, R., Koba, H., Kanda, K. and Shimanuki, T.: "Feeding evasion for rice grassh opper (Oxya yezoensis) of Ephelis-endophyte infected knotgrass (Paspalum thunbergii)"Grassland Science. 46. 74-76 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Fungal endophytes with enhanced plant resistance to insects and their host specificity"IGE (Institute of Genetic Ecology) Series. 26. 145-157 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Backscattered electron imaging of colloidal gold-labeled hyphal surface of Neotyphodium endophytes in perennial ryegrass tissues"J. Electr. Microsc. Technol. Med. Biol.. 16(1). 43-45 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Akiyama, F., Tsukiboshi, T. and Nakayachi O. ; Post-embedding immunogold labelling of Neoryphodium endophyte"J. Electr. Microsc. Technol. Med. Biol.. 16(2). 41 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Recent studies of microbial endophytes and thier prospect"Plant Protection. 55. 475-478 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H., Kurihara, T., Christensen, M.J., Bennett, R.J. and Nakayachi, O.: "Analysis of inteface of Neotyphodium-endophyte/grass interactions by energy-filtering electron microscopy"J. Electr. Micros. Technol. Med. Biol.. 17(in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga, H.: "Microbial endophyte"Riken Green, Tokyo. 1-90 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 古賀博則: "エンドファイト(植物内部共生菌)のコロイド金抗体法を用いたSEM検出"医学生物学電子顕微鏡技術学会誌. 16. 43-45 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 古賀博則, 秋山典昭, 中谷内修: "Post-embedding法による免疫電顕のNeotyphodiumエンドファイト(植物内生菌)への応用"医学生物学電子顕微鏡技術学会第17回学術講演会予稿集. 40 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 古賀博則: "エンドファイト研究の現状と展望"植物防疫. 55. 475-478 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 古賀博則, 栗原孝行, M.T.Christensen, 中谷内修: "Neotyphodiumエンドファイトと寄生植物との接触界面のEF-TEMによる解析"医学生物学電子顕微鏡技術学会第18回学術講演会予稿集. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 古賀博則,中谷内修: "トールフェスク及びペレニアルライグラス組織内のNeotyphodium エンドファイト菌系の免疫電子顕微鏡法による検出"日本植物病理学会報. 66. 179 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀博則: "エンドファイト(植物内部共生菌)のコロイド金抗体法を用いたSEMによる検出"医学生物学電子顕微鏡技術学会第8回医学生物学部電顕シンポジウムプログラム予縞集. 17 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koga,H.: "Ultrastructure of pathogenic and endophytic fungi/plant interfaces."Proceedings of 7th Symposium of mycological Society of Japan. 45-48 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀博則,秋山典昭,中谷内修: "post-embedding 法による免疫電顕のNeotyphodium エンドファイト(植物内生菌)への応用"医学生物学電子顕微鏡技術学会第17回学術講演会および総会プログラム・予稿集. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀博則: "トールフェスク及びペレニアルライグラス組織内のNeotyphodiumエンドファイト菌糸の免疫電子顕微鏡法による検出"2000年度日本植物病理学会大会 プログラム・講演要旨集. 198 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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