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Construction of library of useful nitrile hydratase for various industrial needs

Research Project

Project/Area Number 11660080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionGIFU UNIVERSITY

Principal Investigator

NAGASAWA Toru  Faculty of Engineering, GIFU UNIVERSITY Professor, 工学部, 教授 (60115904)

Co-Investigator(Kenkyū-buntansha) MITSUKURA Koichi  Facultry of Engineering, GIFU UNIVERSITY Assistant Professor, 工学部, 助手 (70324283)
YOSHIDA Toyokazu  Faculty of Engineering, GIFU UNIVERSITY Associate Professor, 工学部, 助教授 (90220657)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsNitrile hydratase / Amycolatopsis strains / Actinomadura strains / Heat tolerance / Organic solvent tolerance / Biocatalyst / 耐熱性酵素 / 工業用生体触媒 / 工業用触媒 / ニトリル分解菌
Research Abstract

The biocatalyst works very efficiently and specifically under mild reaction conditions. The production process using the biocatalyst is often evaluated to be environmentally acceptable production process. The conventional chemical industry has often brought about the severe environmental pollution, because toxic heavy metal catalysts are often used under high pressure or strong acidy or basic conditions. Toxic organic solvents including halogenated organic solvent such as chloroform and dichloromethane have been often used. Thus the introduction of biocatalyst into chemical industry is expected to be realized in the 21 centuries. Nitrile hydratase is very useful and promising biocatalyst for the chemical industry. Rhodococcus rhodochrous J1 nitrile hydratase is used for the industrial production of acrylamide and nicotinamide. However, J1 is not always perfect for any situation. Further specific nitrile hydratase is required for each futher industrial uses. For example, more heat stable, more pH stable and more solvent tolerant nitrile hydratase is required. Nitrile hydratase which works at higher concentration of nitriles and amides, and catalyzes in the presence of cyanide ions, are also necessary. Enantioselective nitrile hydratase has also great demand. Thus, we have started the new survey of novel nitrile hydratases through the new enrichment culture technique and new screening procedures. After all, we have succeeded to isolate the novel nitrile hydratase producing strains, which were identified to be Amycolatopsis and Actinomadura strains. We have purified and characterized these strains. In addition, we also isolated further several unique nitrile hydratase producing strains. Although their activity is still low at present, their unique characterization seems to be very interesting and promising for the industrial application.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] M.Wieser et al.: "Low-molecular mass nitrile hydratase from Rhodococcus rhodochrous J1"FEMS Microbiol.Lett.. 169. 17-22 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nagasawa et al.: "Nitrilase of Rhodococcus rhodochrous J1 : Conversion into the active form by subunit association"European Journal of Biochemistry. 267(1). 138-144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Wieser & T.Nagasawa: "Stereoselective Bioconversion "Stereoselective Nitrile Converting Enzymes"Marcel Dekker, Inc.New York. 35 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Wieser et al.: "Low Molecular mass nitrile hydratase from Rhodococcus rhodochrous J1"FEMS Microbiol.Lett.. 169. 17-22 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nagasawa et al.: "Nitrilase of Rhodococcus rhodochrous J1 : Conversion into the active form by subunit association"European Journal of Biochemistry. 267(1). 138-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Wieser & T.Nagasawa: "Stereoselective Nitrile Converting Enzymes"Stereoselective Bioconversion, Marcel Dekker, Inc., New York. 35 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Wieser et al.: "Low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1"FEMS Microbiol.Lett.. 169. 72-42 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nagasawa et al.: "Nitrilase of Rhodococcus rhodochrous J1:Conversion into the active form by subunit association"European Journal of Biochemistry. 267(1). 138-144 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nagasawa et al.: "Occurrence of novel nitrile hydratase in Amycolotopsis"Archieves of Microbiology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Wieser & T.Nagasawa: "Stereoselective Bioconversion"Stereoselective Nitrile-Converting Enzymes"Marcel Dekker,Inc.New York. 35 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Wieser et al.: "Low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1:purification,substrate specificity and comparison with the analogous high-molecular-mass enzyme"FEMS Microbiology Letters. 169. 17-22 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Nagasawa et al.: "Nitrilase of Rhodococcus rhodochrous J1:Conversion into the active form by subunit association"European Journal of Biochemistry. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Wieser et al.: "Stereoselective Bioconversion"Stereoselective Nitrile-Converting Enzymes"Marcel Dekker,Inc.. 35 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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