| Project/Area Number |
11660089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YAMAMOTO Kenji Graduate School of Biostudies, Kyoto University, Professor, 生命科学研究科, 教授 (70109049)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAKI Hisanori Graduate School of Biostudies, Kyoto University, Assistant, 生命科学研究科, 助手 (20212045)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Lactic acid bacteria / Lactobacilli / Immune activation / Immunoglobulin A / Mucosal immunity / Peyer's patch |
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| Research Abstract |
The purpose of this work is to elucidate whether Lactobacilli cells show high activation of the mucosal immune system or not. We investigated the IgA antibody response of mouse intestine by Lactobaciili cells.
At first, we established the assay method of the secretory IgA using anti-IgA antibody. Then, we isolated one Lactobacilli strain from human feces using specific culture media. We identified this Lactobacilli strain as Lactobacillus pantarum by sugar assimilation test. When the isolated strain was orally administrated to BALB/c mouse, the IgA concentration of the intestinal fluid of the mouse was significantly increased.
Next, in vitro IgA production assay was done using Peyer's patch cells of mouse. The isolated strain was examined as to the response of Peyer's patch cells of mouse and showed the high immunopotentiating activity. We also confirmed that the cell wall fraction of the isolated strain which was obtained by sonication showed comparatively high activity.
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