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Regulation of chromosome segregation in the budding yeast, S.cerevisiae

Research Project

Project/Area Number 11660093
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionHiroshima University

Principal Investigator

TSUCHIYA Eiko  Hiroshima University, Graduate School of Advanced Sciences of Matter, professor, 大学院・先端物質科学研究科, 教授 (90127671)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsSaccharomyces cerevisiae / chromosome segregation / protein kinase C / microtuble-binding protein / chromatin-remodeling complex / S.cerevlsiae / S,cerevisiae / クロマチン構造変換 / RSC / NPSI / STHI
Research Abstract

NPS1/STH1 encodes a catalytic subunit of RSC and plays essential role for mitotic growth. Our previous analysis on temperature-sensitive mutant allele of NPS1, nps1-105, revealed that Nps1/RSC plays important role on the assembly and/or maintenance of the chromatin structure around centromeres. We performed this study on an attempt to understand the in vivo function of RSC and obtained the following results.
1) We isolated fifteen suppressor mutants (rtn mutants) that can restore the growth of nps1-105 in the presence of TBZ.These mutants were classified in nine complementation groups. Taking an advantage of temperature sensitivity, we cloned RTN2, RTN4 and RTN5 and identified them as NUP82, ABD1 and CET1, respectively.
2) We screened high-copy suppressor genes for the temperature- and thiabendazole (TBZ) nps1-105 mutant and identified nine genes including NPS1. Among those, PKC1 and BIM1 that respectively encoded a homolog of mammalian protein kinase C and a conserved microtubule binding protein homologous to human EB1 were found to play important role on suppression. Genetic analysis of the functional relationships between these genes revealed that PKC1 suppressed the defect of nps1-105 via the function of BIM1 but not through activation of the MPK1/MAPK pathway. The stt1 mutation showed TBZ sensitivity and delayed G2-phase progression at semi-permissive temperatures. Both of these stt1 phenotypes were suppressed by the overexpression of BIM1. In addition, stt1 as well as nps1-105, mis-segregated a mini-chromosome at higher frequency than the wild type at permissive temperature. The mis-segregation was enhanced in the nps1-105 stt1 double mutant. These results suggested that Pkc1p plays a role relevant to microtubule function and that this role is mediated by an unknown PKC effector branch and by Bim1p.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] M.Yukawa: "Nps1/Sth1p.a component of an essential chromatin-remodeling complex of Saccharomyces cerevisiae, is equired for the maximal expression of early-meiotic genes"Genes to Cells. 4巻・1号. 99-110 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.C.Farcasanu: "Involvement of thioredoxin peroxidase typeII (Ahp1p) of Saccharomyces cerevisiae in Mn2+ homeostasis"Biosci.Biotechnol.Biochem.. 63巻. 1871-1881 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Tsuchiya: "Borrelidin inhibits a cyclin-dependent kinase (CDK), Cdc28/Cln2, of Saccharomyces cerevisiae"J.Antibiotics. 54巻・1号. 84-90 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Yukawa: "Nps1/Sth1p, a component of an essential chromatin-remodeling complex of Saccharomyces cerevisiae, is required for the maximal expression of early-meiotic genes"Genes to Cells. vol.4. 99-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.C.Farcasanu: "Involvement of thioredoxin peroxidase typeII (Ahp1p) of Saccharomyces cerevisiae in Mn2+homeostasis"Biosci.Biotechnol.Biochem. vol.63. 1871-1881 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Tsuchiya: "Borrelidin inhibits a cyclin-dependent kinase (CDK), Cdc2 8/Cln2, of Saccharomyces cerevisiae"J.Antibiotics. vol.54. 84-90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Tsuchiya 他: "Borrelidin inhibits a cyclin-dependent kinase(CDK), Cdc28/Cln2, of Saccharomyces cerevisiae"J.Antibiotics. 54巻・1号. 84-90 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Yukawa: "Nps1/Sth1P,a component of an essential chromatin-remodeling complex of Saccharomyces cerevisiae,is required for the maximal expression of early-meiotic genes"Genes to Cells. 4・1. 99-110 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.C.Forcasanu: "Involvement of thioredoxin peroxidase typeII(Ahp1p)of Saccharomyces cerevisiae in Mn2+homeostasis"Bioscience Biotechnology and Biochemistry. 63・11. 1871-1881 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 土屋: "バイオテクノロジー講義"朝倉書店. 156 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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