| Project/Area Number |
11660104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
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| Research Institution | NIIGATA UNIVERSITY |
|---|
Principal Investigator |
HOSHINO Tsutomu DEPARTMENT OF APPLIED BIOLOGICAL CHEMISTRY, FACULTY OF AGRICULTURE, NIIGATA UNIVERSITY, PROFESSOR, 農学部, 教授 (30165542)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | squalene / oxidosqualene / triterpene / hopene / lanosterol / cyclase / site-directed mutagenesis / Alicyclobacillus acidocaldarius |
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| Research Abstract |
This investigation was carried out to gain insight into the squalene cyclization mechanism by using the substrate analogs and by site-directed mutagenesis experiments. We established the initiation site of the cyclization and the stabilization sites as follows. The amino acid alignment of DXDDTA motif, the acidity being enhanced by His 451, is responsible for the initiation and Phe365, Phe601 and Phe605 residues stabilize the cationic intermediates. Incubation experiments of 10-desmethylsqualene analog showed the methyl group at 10-position is most critical to the completion of squalene cyclization.
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