| Project/Area Number |
11660105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Shizuoka University |
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Principal Investigator |
USUI Taichi Shizuoka University, Faculty of Agriculture, Professor, 農学部, 教授 (50111802)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Takeomi Shizuoka University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (30273171)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Cell surface model / Carbohydrate interaction / Oligosaccharide unit / Molecular recognition / Simulation / Glycopolymer / Glycotechnology / Glycoconjugate / Carbohydrate interaction / Glycoll / Glycoprotein / Glycolipid / Glycotechnology / Carbohydrate probe / Oligosaccharide unit / Glycopolymer / Carbohydrate intraction / Oligosaccharide ligand / Oligosaccharide synthesis |
|---|
| Research Abstract |
Biologically functional carbohydrates exist on cell surface and play important roles on the intercellular interaction in cell-cell recognition. We developed a simple enzymatic method of the artificial neoglycopeptide with a poly(L-glutamic acid)s backbone carrying multivalent Lex as a mimic of the embryoglycan and the sialoglycopeptides carrying multivalent sialyl oligosaccharides as mimics of mucin-like glycopeptides. The former synthetic glycopeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of interaction with lectins by using a surface plasmon resonance analyzer BIAcore. The multiplicity of oligosaccharide as side chain was very important to induce the affity enhancement glycoprotein-lectin interaction. The latter glycopeptides were elucidated to be very useful for invesrigating the sialic acid (SA) linkage specific neutralization of influenza type A and B virus strains. It indicated that the multivalent sialyl sugar units in the neoglycopeptides drastically increase the viral neutralizing activities.
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