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Simulation of functional glycomolecule as carbohydrate model on cell surface

Research Project

Project/Area Number 11660105
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionShizuoka University

Principal Investigator

USUI Taichi  Shizuoka University, Faculty of Agriculture, Professor, 農学部, 教授 (50111802)

Co-Investigator(Kenkyū-buntansha) MURATA Takeomi  Shizuoka University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (30273171)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCell surface model / Carbohydrate interaction / Oligosaccharide unit / Molecular recognition / Simulation / Glycopolymer / Glycotechnology / Glycoconjugate / Carbohydrate interaction / Glycoll / Glycoprotein / Glycolipid / Glycotechnology / Carbohydrate probe / Oligosaccharide unit / Glycopolymer / Carbohydrate intraction / Oligosaccharide ligand / Oligosaccharide synthesis
Research Abstract

Biologically functional carbohydrates exist on cell surface and play important roles on the intercellular interaction in cell-cell recognition. We developed a simple enzymatic method of the artificial neoglycopeptide with a poly(L-glutamic acid)s backbone carrying multivalent Lex as a mimic of the embryoglycan and the sialoglycopeptides carrying multivalent sialyl oligosaccharides as mimics of mucin-like glycopeptides. The former synthetic glycopeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of interaction with lectins by using a surface plasmon resonance analyzer BIAcore. The multiplicity of oligosaccharide as side chain was very important to induce the affity enhancement glycoprotein-lectin interaction. The latter glycopeptides were elucidated to be very useful for invesrigating the sialic acid (SA) linkage specific neutralization of influenza type A and B virus strains. It indicated that the multivalent sialyl sugar units in the neoglycopeptides drastically increase the viral neutralizing activities.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Tateomi Murata: "Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto-N-neofetraose"Glycoconjugate J.. 16. 189-195 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Xianoxing Zeng: "Chemoenzymatic synthesis of glycopolypeptides carrying α-Neu5Ac-(2-3)-β-D-Gal-(1-3)-α-D-GalNAc, and related compounds"Arch. Biochem. Biophys.. 383. 28-37 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tateomi Murata: "Enzymatic synthesis of important oligosaccharide units involved in N-and O-linked glycans"Trends in Glycosci. Glycotechnol.. 12. 161-174 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tateomi Murata: "Enzymatic synthesis of sulfated disaccharides using β-D-galactosidase-catalyzed transglycosylation"Biosci. Biotechnol. Biochem.. 65. 2456-2464 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuhide Totani: "Enzymatic synthesis of aliphatic β-lactosides as mimic units of glycosphingolipids by use of T.reesei cellulase"Arch. Biochem. Biophys.. 383. 28-37 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuhide Totani: "Enzymatic synthesis of oligosaccharide containing Le^X unit using partially purified chicken serum"Biosci. Biotechnol. Biochem.. 66. 636-640 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Totani, K. Shimizu, Y. Harada, T. Murata, T. Usui: "Enzymatic synthesis of oligosaccharide containing Lex unit by partially purified chicken serum"Biosci. Biotechnol. Biochem.. 66. 636-640 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Murata, M. Kosugi, T. Nakamura, T. Urashima, T.Usui: "Enzymatic synthesis of sulfated disaccharides using β-D-galactosidase-catalyzed transglycosylation"Biosci. Biotechnol. Biochem.. 65. 2456-2464 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Totani, N. Yasutake, H. Ohi, T. Murata, T. Usui: "Enzymatic synthesis of aliphatic b-lactosides as mimic units of glycosphingolipids by use of Trichoderma reesei"Arch, Biochem. Biopys.. 383. 28-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] X. Zeng, Y. Nakaaki, T. Murata, T. Usui: "Chemo-enzymatic synthesis of glycopeptides carrying α-Neu5Ac-(2-3)-β-D-Gal-(1-3)-α-D-GalNAc, β-D-Gal-(1-3)-α-D-GalNAc and related compounds and analysis of their specific interactions with lectins"Arch. Biochem. Biopys.. 383. 28-37 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T .Murata, T. Usui: "Enzymatic synthesis of important oligosaccahride units in N- and O-linked glycans"Trends Glycosci. Glycotechnol.. 12. 161-174 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Murata, T. Inukai, T. Suzuki, S. M. Yamagishi, T. Usui: "Facile enzymatc conversion of lactose into lacto-N-tetraose and lacto-N-tetraose"Glycoconjugate J.. 16. 189-195 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuhide Totani: "Enzymatic synthesis of aliphatic β-lactosides as mimic units of glycosphingolipids by use of Trichoderm reesei"Arch. Biochem. Biophys.. 383. 28-37 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kazuhide Totani: "Enzymatic synthesis of oligosaccharide containing Lex unitby partially purified chicken serum"Biosci. Biotechnol. Biochem.. 66. 636-640 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 村田健臣: "機能性オリゴ糖の構築"日本農芸化学会. 7.5. 576-579 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Xiaoxiong Zeng: "Regioselective synthesis of p-nitropheyl glycoside of β-D-galactopyranosyl-disaccharide by use of fransglycosylation"Carbohydr,Res.. 325. 120-131 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Xianoxiong Zeng: "Chemoenzymatic synthesis of Glycopolypeptides carring α-NeuSAc-(2-3)-β-D-Gal-(1→3)-d-D-GalNAc related compounds"Arch.Biochem.Biophys.. 383. 28-37 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hisashi Ashida: "Characterization of endo-N-acetylgalactosaminidase from Bacillus sp.and synthesis of neo-oligosaccharides using its transglycosylation"Arch.Biochem.Biophys.. 373. 394-400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeomi Murate: "Ensymatic synthesis of important oligosaccharide unito involved in N-and O-linked glycans"Trends in Glycosci Glycotechnol.. 12. 161-174 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazuhide Totani: "Enzymatic synthesis of aliphatic β-loctosides as mimic units of glycosphingolipids by use of Trichoderma reesei cellulase"Arch.Biochem.Biophys.. 385. 70-77 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zeng,X,.Murata,T.,Kawagishi,H,Usui,T.,and Kobayasfli K.: "Analysis of specific interactions of synthetic glycopolypeptides carrying N-acetyllactosamine and related compounds with lectins"Carbohydr.Res.. 312. 209-217 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murata,T.,Inukai,T.,Suzuki,M.,Yamagishi,M and Usui,T: "Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto -N - neotetraose"Glycoconjugate.J,. 16. 189-195 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murata,T.,Morimoto,S.,Zeng,X,.Watanabe,S.,and Usui,T: "Enzymatic synthesis of O-α-L-fucosyl N-acetyllactosamine and 3'-O -α-L-fucosyllactose utilizing α- L- fucosidases"Carbohydr.Res.. 320. 192-199 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zeng,X,Yosino R.,Murata,T.,Ajisaka K.,and Usui,T: "Regioselective synthesis of p-nitrophenyl glycoside of β-D -galactopyranosyl-disaccharide by use of transglycosylation"Carbohydr.Res.. 325. 120-131 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ashida H.,Yamamoto K.,Murata,and Usui,H.: "Characterization of endo-N-acetygalactosaminidase from Bacillus sp.and symtheses of neo-olligosaccharides using its transglyocosylation"Arch.Biochem.Biophys.. 373. 394-400 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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