| Project/Area Number |
11660111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
NAKAJIMA Hiromitsu Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (40144646)
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| Co-Investigator(Kenkyū-buntansha) |
中島 廣光 鳥取大学, 農学部, 教授 (40144646)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | plant pathogens / phytotoxins / biosynthesis / Bipolaris sorokiniana / sesquiterpene / ^<14>C labeled compound / ^3H labeled compound / GCMS / ^<18>O標識化合物 |
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| Research Abstract |
Sorokinianin is a phytotoxin produced by a strain (OB-25-1) of Bipolaris sorokiniana, a pathogen causing spot blotch or foot and root rot diseases of wheat and other grasses. This toxin is assumed to be composed of a helminthosporol-type sesquiterpene and a C_3 portion. The feeding experiments conducted previously suggested that sorokinianin was synthesized from prehelminthosporol by reactions that include the addition of a member of the TCA cycle, decarboxylation and lactonization. A feeding experiment with L-[hydroxy-^<18>O] malic acid indicated that the direct C_3 unit precursor was not malic acid/oxalacetic acid but fumaric acid/succinic acid. The aim of this research is to identify the product formed just after addition of fumaric acid/succinic acid to prehelminthosporol. For this purpose we prepared ^<14>C labeled prehelminthosporol (1.7 mg, specific radioactivity 35 KBq/mg) from B.sorokiniana cultured on the medium containing ^<14>C labeled glucose. Among the fractions obtained from the mycelium incubated with both ^<14>C labeled prehelminthosporol and ^3H labeled succinic acid we found a fraction which had both ^<14>C and ^3H radioactivity. Then we analyzed the same fraction obtained in the cold run as in the radio isotopic experiment by GCMS after acetylation and methylation.
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