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Bioremediation of dioxins with dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi

Research Project

Project/Area Number 11660166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionEhime University

Principal Investigator

TACHIBANA Sanro  Ehime University, Faculty of Agriculture, Professor, 農学部, 教授 (10112319)

Co-Investigator(Kenkyū-buntansha) NINOMIYA Ikuo  Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80172732)
ITOH Kazutaka  Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50253323)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsDioxins / Screening / Bioremediation / Protoplast fusion / Incinerated ashes / Contaminated soils / Purification of Environment / Dioxins degradable fungi / 土壌
Research Abstract

Biodegradation of dioxins in soils and incinerated ashes by bioremediation was conducted by use of dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi. And, formation of fusants having higher ability for degradation of dioxins formed by the fusion of the fungi were also conducted.
1. Each dioxin (2, 7-DCDD, 2, 4, 8-TCDF and 2, 3, 7, 8-TCDD) in soils could be degraded by bioremediation with dioxins degradable fungi. Each dioxin was degraded about 70%, 65% and 60%, respectively, for 30 days.
2. About 90% of dioxins in paddy filed soils was degraded by bioremediation with dioxins degradable fungi for 30 days. The toxicity of the dioxins in the solis was alos removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased by addition of a surfactant during the bioremediation.
3. About 50% of dioxins in nicinerated ashes was degraded by bioremediation with dioxins degradable fungi for 60 days. It was found that the degree of the dioxins in the ashes was considerably increased by repetition of the bioremediation.
4. Dioxins in paddy filed soils could be degraded by bioremediation with fusants formed by prptoplast fusion of the fungi. The degree of degradation of the dioxins was a little higher than that by the bioremediation with the fungi. In addition, the ability of fusants for degradation of dioxins decreased in the repeated subcultures.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 大川浩樹: "木材腐朽菌によるバイオレメティエーション(III)-ダイオキシン分解菌による2,4,8-トリクロロジベンゾフラン及びジベンゾフランの分解"紙パ技協誌. 53. 1054-1062 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 橘燦郎: "ダイオキシン汚染土壌の木材腐朽菌による生物処理"用水と廃水. 41. 698-705 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyota Yukinori: "Degradation of dioxins (2,7-dichlorodibenzo-p-dioxin) by some fungi in a natural soil"Proceedings of 10th International Symposium on Wood and Pulping chemistry. III. 102-105 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyota Yukinori: "Biodegradation of dioxins by fungi having ability for degradation of dioxins"Proceedings of International Symposium on Environmental Friendly and Emerging Technologies for Sustainable Pulp. 347-350 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 古賀道慶: "ダイオキシン分解能を有する菌による焼却灰中のダイオキシン類の分解"第45回リグニン討論会講演集. 199-200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 安本さいか: "細胞融合によるダイオキシン分解能を有する融合菌の作出の試み"第50回日本木材学会大会研究発表要旨集. 678-678 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohkawa Hiroki et al.: "Bioremediation by Wood-Rotting fungi (III) Degradation of 2, 4, 8-trichlopodibenzofuran and dibenzofuran by some fungi having ability for degradation of dioxins."Japan Tappi. Vol.53. 1054-1062 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tachibana Sanro: "Bioremediation of dioxins contaminated soils with white-rotting fungi."Journal of Water and Waste. Vol.41. 698-705 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyota Yukinori et al.: "Degradation of dixoins (2, 7-dichlorodibenzo-p-dioxin) by some fungi in a natural soil."PROCEEDINGS OF 10TH INTERNATIONAL SYMPOSIUM ON WOOD AND PULPING CHEMISTRY, Yokohama. Vol. III. 102-105 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyota Yukinori et al.: "Biodegradation of dioxins by fungi having ability for degradation of dioxins."PROCEEDINGS OF INTERNATIONAL SYMPOSIUM ON ENVIRONMENTAL FRIENDLY AND EMERGING TECHNOLOGIES FOR SUSTAINABLE PULP AND PAPER INDUSTRY, Taipei. 347-350 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koga Michifusa et al.: "Bioremediation of dioxins in incinerated ashes by fingi having ability for degradation of dioxins."PROCEEDINGS OF THE 45TH LIGNIN SYMPOSIUM, Matsuyma. 199-200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasumoto Saika et al.: "Formation of fusants having higher ability for degrdation of dioxins by protoplast fusion of fungi."ABSTRACTS OF THE 50th ANNIVERSARY CONFERENCE OF THE JAPAN WOOD RESEARCH SOCIETY, Kyoto. 678 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 清田幸徳: "天然からスクリーニングした菌によるダイオキシン類のバイオレメディエーション"第50回日本木材学会大会研究発表要旨集. 408-408 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀通房: "数種のダイオキシン分解菌によるダイオキシンの分解"第50回日本木材学会大会研究発表要旨集. 407-407 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 清田幸徳: "天然からスクリーニングしたダイオキシン類のバイオレメディエーション(II)"第45回リグニン討論会講演集. 197-198 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀道房: "ダイオキシン分解能を有する菌による焼却灰中のダイオキシン類の分解"第45回リグニン討論会講演集. 199-2000 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kiyota Yukinori: "Biodegradution of dioxins by fungi having ability for degradation of dioxins."International Symposium on Environmentally Friendly and Emerging Technologies for a Sustainable Pulp and Paper Industery. 347-350 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀通房: "数種のダイオキシン分解菌によるダイオキシンの分解"第49回日本木材学会大会研究発表要旨集. 373-373 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Koga Michifusa.: "Screening of fungi having ability for degradation of dioxing"10th International Symposium on Wood and Pulping Chemistry. 3. 98-101 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kiyota Yikinori: "Degradation of dioxins〔2,7-dichlorodibenzo-P-dioxin〕by some fungi in a natural soil"10th International Symposium on Wood and Pulping Chemistry. 3. 102-105 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 橘 燦郎: "ダイオキシン汚染土壌の木材腐朽菌による生物処理"用水と廃水. 41・8. 698-705 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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