Efficacy of anti-viral interferon to fish viral diseases

• Project/Area Number: 11660186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Mie University
• Principal Investigator: MIYAZAKI Teruo Mie University, Faculty of Bioresources, Professor, 生物資源学部, 教授 (20046345)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000); Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: Japanese pearl oysters / Japanese oysters / A recombinant feline interferon / rFeIFN-receptor / ウイルス性外套膜緑色化症 / 大量死 / アコヤウイルス / rFeIFN-ω投与 / 治療効果

Research Abstract

Many new viral diseases occur and caused great economic losses in cultured fishes, Japanese pearl oysters and Japanese oysters.In the present study, I examined the clinical efficacy of administration of a recombinant feline interferon (rFeIFN) to protect Japanese pearl oysters from akoya-virus infection and Japanese oysters from 'Viral green mantle disease' (VGMD). The good efficacy resulted in treating Japanese pearl oysters that were artificially infected with cultured akoya-virus. On the other hand, the rFeIFN administration to Japanese oysters that were infected with cultured VGMD-virus resulted in lesser efficacy in prophylaxis and treatment.
Moreover, I examined the rFeIFN-receptor on hemocytes of pearl oysters and Japanese oysters because hemocytes work for phagocytizing viruses and preventing the virally-infected lesions from development. Almost 56% of agranulocytes of pearl oysters possessed the rFeIFN-receptor on the cytoplasms. Therefore, in pearl oyster, agranulocytes with the rFeIFN-receptor were enhanced the phagocytic potential by rFeIFN.On the other hand, only 7.3% agranulocytes of Japanese oysters possessed the rFeIFN-receptor, which caused lesser efficacy.

Research Products

• [Publications] T.Miyazaki et al.: "Mass mortalities associated with avirus disease in Japanes pearl oysters"Diseases of Aquatic Organisms. 37. 1-12 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Miyazaki et al.: "Viremia-associated Ana-aki-byoc A New viral disease in calor carp in Japan"Diseases of Aquatic Organisms. 39. 183-192 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Miyazaki et al.: "Clinical trial results on the use of rFeIFN-ω to protect Japane searl oyster"Diseases at Aquatic Organisms. 43. 15-26 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Teruo Miyazaki: "Mass Mortalities Associated with A Virus Disease in Japanese Pearl Oysters Pinctada fucata martensii."Diseases of Aquatic Organisms. 37. 1-12 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Teruo Miyazaki: "Viremia-associated Ana-aki-byo, A New Viral Disease in color carp Cyprinus carpio in Japan."Diseases of Aquatic Organisms. 39. 183-192 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Teruo Miyazaki: "Clinical trial results on the use of a recombinant feline interferon-ω to protect Japanese pearl oysters Pinctada fucata martensii from akoya-virus infection."Diseases of Aquatic Organisms. 43. 15-26 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.Miyazaki: "Mass Mortarities associated wich a virus disease in Japanes pearl oyster"Diseases of Aquatic organisms. 37(1). 1-12 (1999)
• [Publications] T.Miyazaki: "Viremia-associatd Ana-aki-byo, A new viral disease in color carp-in Japan"Diseases of Aquatic Organisms. 39(3). 185-192 (2000)
• [Publications] T.Miyazaki: "Clinical trial results on use of a recombinant feline in terferon-w to protect Japane peoroyster"Diseases of Aquatic Organisms. 43(1). 15-26 (2000)
• [Publications] T.Miyazaki: "Mass mortality associated with a virus disease in Japanese pearl oysters pinctada fucata martensii"Diseases of Aquetic organisms. 37. 1-12 (1999)