| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
In the first year, we have demonstrated lipid hydroperoxide (LPO) and hydroxy lipid levels in the muscles and livers of normal or CC14 -injected live fish In general, LPO levels in vivo reflect the physiological conditions induced by oxidative stress (see reference! ).
In the second year, LPO and hydroxy lipid levels of diseased fishes were investigated. Although significant differences in LPO levels were not always observed between normal and diseased fish, hydroxy lipid levels in muscles and liver were significantly higher in diseased fish than in uninfected normal fish cultured with the same feed at the same place or in wild normal fish. TG-OH levels of diseased yellowtails with streptococcal infection were significantly high in ordinary and dark muscle, especially in liver ; 1072±78 in diseased fish compared to 632±45 (nmol/g tissue) in normal fish cultured with the same feed. In this case, the polyunsaturated fatty acid contents of the diseased fish were decreased ; notably, the decrease of DHA (22 : 6, n-3) was remarkable. These phenomena, increased accumulation of hydroxy lipids in liver of diseased fish and decreased levels of polyunsaturated fatty acids, were in sweetfish (ulcer disease), globefish (Heterobothrium okamotoi infection and/or emaciation disease), red sea bream (edwardsiellosis and/or Vibrio anguillarum), yellowtail (jaundiced disease), and carp (cold water disease).
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