Isolation and identification of inhibitors against matrix metallo proteinase in shark cartilage

• Project/Area Number: 11660208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SATO Kenji Faculty of Human Environment, Kyoto Prefectural University, Department of Food Sciences and Nutritional Health, Associate Professor, 人間環境学部, 助教授 (00202094)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: Shark / MMP / Inhibitor / Collagenase / Gelatinase / Muco polysaccharide / MMP-9 / Cancer / マトリックスメタロプロテアーゼ / サメ / 軟骨 / 多糖類 / Autofocusing

Research Abstract

Matrix metallo proteinases (MMP), especially MMP-9 plays a significant role in invasion and metastasis of cancer. An objective of the present research project is to isolate and identify the material having the inhibitory activity against MMP-9. The crude water-extract of dried shark fin had an inhibitory activity against MMP-9. The activity was concentrated into an acidic fraction by preparative isoelectric focusing in the absence of added ampholyte. In addition, it was recovered in the non-adsorbed fraction of the reversed-phase HPLC and void volume fraction of the size exclusion chromatography. A compositional analysis revealed that it consists of uronic acid and galactsamine. These facts indicate that the acidic muco polysacchmide in the shark cartilage has the inhibitory activity against MMP-9. Then the activity can be easily concentrated as an ethanol (75%) insoluble fraction of the water extract. Now therapeutic effect of the ethanol insoluble fraction by oral administration is examined by using an animal model, chemically-induced pancreatic cancer in hamster.

Research Products

• [Publications] 赤星亜寿香: "Novel approach for large-scale, biocompatible, and low-cost fractionation of peptides in proteolytic digest of food protein based on the amphoteric nature of peptides"J.Agric.Food.Chem.. 48・5. 1955-1959 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Asuka Akahoshi, Kenji Sato, Yuko Nawa, Yasushi Nakamura, and Kozo Ohtsuki: "A Novel Approach for Large Scale, Biocompatible and Low Cost Fractionation of Peptides in Proteolytic Digest of Food Protein on the Basis of Amphoteric Nature of Peptides."Jouranal of Agricultural and Food Chemistry. 48 (5). 1955-1959 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 赤星亜寿香: "Novel approach for large-scale, biocompatible, and low-cost fractionation of peptides in proteolytic digest of food protein based on the amphoteric nature of peptides"J.Agric Food Chem.. 48・5. 1955-1959 (2000)
• [Publications] Asuka Akahoshi, Kenji Sato, Yuko Nawa, Yaushi Nakamura, Kozo Ohtsuki,: "A novel approach for largo scalo, biocompatiblo and low cost fractionation of peptide In proteolytic digoat of food protein on the basis of amphoteric nature of peptide"Journal of Agricultural and Food Chomistry. (印刷中). (2000)