| Project/Area Number |
11660282
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
KITA Kazumi Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Associate Professor, 大学院・生命農学研究科, 助教授 (20221913)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | IGFBP-2 / Gizzard / Brain / Dietary protein / Amino acid / Gene expression / Insulin / Chickens / 大豆抽出蛋白質 / カゼイン / インスリン様成長因子 / インスリン様成長因子結合蛋白質 |
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| Research Abstract |
The influence of feeding with experimental diets containing different dietary protein quantity and quality on changes in IGFBP-2 gene expression in various tissues of young chickens was examined. The hepatic IGFBP-2 mRNA was not detected among all treatments. When chickens fed a 5% soybean diet, brain IGFBP-2 mRNA level was lower than that of chickens fed a 20% soybean diets supplemented with essential amino acids. In the gizzard, IGFBP-2 mRNA in the group of a 20% soybean diets supplemented with essential amino acids was significantly lower than other groups. There was no significant difference in IGFBP-2 gene expression in the kidney among all treatments. These results suggest that the difference in dietary protein quantity affect IGFBP-2 gene expression in brain and gizzard, and that the difference in dietary protein quality affects IGFBP-2 gene expression in only gizzard. The expression of IGFBP-2 gene might be less responsive to the difference in dietary protein quality than that in dietary protein quantity.
The changes in tissue IGFBP-2 gene expression of young fasted chickens followed by refeeding a commercial diet or by administrating bovine insulin was examined. Hepatic IGFBP-2 mRNA was significantly increased by fasting for 2 days and decreased by refeeding for 6 h. Insulin administration also decreased hepatic IGFBP-2 gene expression stimulated by fasting to the level of refed chickens. IGFBP-2 mRNA in the gizzard was significantly increased by fasting for 2 days and tended to decrease by refeeding for 6 h. Insulin administration decreased gizzard IGFBP-2 gene expression lower than the level of refed chickens. There was no significant difference in IGFBP-2 mRNA in the brain and kidney among all treatments. These results suggest that the changes in IGFBP-2 gene expression in the liver and gizzard by fasting and refeeding might be partly regulated by the alteration in plasma insulin concentration.
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