| Project/Area Number |
11660286
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | TOKYO UNIVERSITY OF AGRICULTURE |
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Principal Investigator |
IWASAKI Setsuo FACULTY OF APPLEED BIO-SCIENCE, PRPOFESSOR, 応用生物科学部, 教授 (50184867)
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| Co-Investigator(Kenkyū-buntansha) |
TAKADA Tatsuyuki SHIGA UNIVERSITY OF MEDICAL SCIENNCE, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助教授 (90206756)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | nuclear transfer / dedifferentiation / totipotency / gene expression / GFP |
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| Research Abstract |
The present study was carried out to clarify the specificity of gene expression during dedifferentiation or acquisition of totipotency in bovine somatic cells. In fact, the preparation of reporter gene, establishment of gene transferred cell lines, production of reconstituted embryos by nuclear transfer using gene transferred donor cells and gene expression of reconstituted embryos were investigated. The following results were obtained. 1. The preparation of reporter genes from GFP vectors inserted with PGK promorter between EcoRI and PstI were obtained successfully. 2. The GFP expression in transfected bovine fibroblast cells with GFP vector was observed 35-55% after 2 and 20 days after selection. 3. The reconstituted embryos were obtained by a nucleat transfer with GFP-positive cells. 4. GFP expression was observed in reconstituted bovine embryos by nuclear transfer with GFP-positive cells from 1-cell to blastocyst stages. These results show that it was possible to introduce the foreign genes into bovine embryos by nuclear transfer and these embryos show the expression of foreign gene in whole embryos.
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