Function of kupffer cells and neutrophils in diabetic rats.

Research Project

Project/Area Number	11660300
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	Osaka Prefecture University
Principal Investigator	MORIYAMA Mitsuaki (2001) Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Research Associate, 農学生命科学研究科, 助手 (20275283) 菅野司 (1999-2000) 大阪府立大学, 農学生命科学研究科, 教授 (30081516)
Co-Investigator(Kenkyū-buntansha)	KANNAN Yukiko Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Assistant Professor, 農学生命科学研究科, 講師 (80264810) 森山光章大阪府立大学, 農学生命科学研究科, 助手 (20275283)
Project Period (FY)	1999 – 2001
Project Status	Completed (Fiscal Year 2001)
Budget Amount *help	¥1,800,000 (Direct Cost: ¥1,800,000) Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords	diabetes / liver injury / Kupffer cell / endotoxin / TNF-a / GPT, GOT / Kuoffer細胞 / GPT, GOT / STZ誘発糖尿病マウス / 好中球 / Zymosan刺激 / アミノグアニジン / TNFα / GOT、GPT
Research Abstract	To clarify the role of kupffer cell in impared defense system in diabetes, we determined the serum levels of GPT, GOT and cytokine after LPS injection in non-insulin-dependent diabetes (NIDDM) model, Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Serum GOT, GPT, TNF-α and IL1-β increased after single injection of LPS, however, these markers were not changed in normal controls, Long-Evans Tokushima Otsuka (LETO) rats. Pretreatment of gadolinium chloride, an inhibitor of the function of kupffer cell, failed to augment these markers in OLETF rats. The number of kupffer cell was increased in OLETF rats. These results indicate that the function of kupffer cells are activated in diabetic rats. This conclusion is confirmed by the fact that glycogenolysis is higher in OLETF rats than that of LETO rats after zymosan treatment.