| Project/Area Number |
11670007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Shinshu Univ., Faculty of Med. |
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Principal Investigator |
MORIIZUMI Tetsuji Shinshu Univ., Faculty of Med., Dept.of Anat.(II) Professor, 医学部, 教授 (70157874)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Takashi Shinshu Univ., Sch.of Allied Medical Sciences Assistant Professor, 医療技術短期大学部, 講師 (50283247)
NAKAYAMA Jun Shinshu Univ., Graduate Sch., Division of Med., Associate Professor, 大学院・医学研究科, 助教授 (10221459)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Cell migration / Neural stem cells / Neuronal differentitation / Olfactory bulb / Calretinin / 神経系乾細胞 / ニューロン形成 / 神経細胞 / 細胞分化 / ポリシアル酸 / ネスチン |
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| Research Abstract |
We investigated neural cell migration through the rostral migratory stream (RMS) in the rat forebrain and obtained the new findings as follows.
(A) Neurogenesis is ongoing in a small population of cells within the RMS during migration. Calretinin-immunoreactive neurons of the RMS co-express nestin (20.8%) and polysialylated neural cell adhesion molecule (61.8%). Calretinin-immunoreactive neurons in the RMS still express immature neural and neuronal phenotypes.
(B) By the BrdU tracing method, newly formed periglomerular cells in the olfactory bulb are mostly replaced during life.
(C) With the combined method of autoradiography using ^3H-thymidine and immunohistochemistry for a neuronal maker, 65.3-76.9% of bulbar neurons, following migration through the forebrain migratory route (RMS), undergo neuronal cell death during six weeks.
(D) By preventing neural cell migration, we demonstrated neurogenesis by local neural stem cells in the olfactory bulb. Further, we revealed differential neurogenesis and gliogenesis by local and migrating neural stem cells in the brain.
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