| Project/Area Number |
11670023
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The Kitasato Institute (2000-2001)
Tokai University (1999) |
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Principal Investigator |
SAKABE Kou ENVIRONMENTAL MEDICAL CENTER, DIRECTOR, 北里研究所病院・アレルギー科, 研究員 (70162302)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | GUT-ASSOCIATED LYMPHOID SYSTEM / ESTROGEN / ENVIRONMENTAL HORMONES / PEYER'S PATCH / LYMPHOCYTES / APOPTOSIS / 7A6 ANTIGEN / IMMUNE DISRUPTQR / 性ホルモン / 環境シグナル / ホルモン様化学物質 / 免疫毒性 |
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| Research Abstract |
The effect of environmental endocrine disruptors (EDC)on the immune organ (Peyer s patch) from castrated female mice was examined by molecular biologic, microscopic and flow cytometric techniques.
Our findings were as follows : (a) using a monoclonal antibody to estrogen receptor-alpha (ER), ER level in the EDC-treated Peyer's patch was almost 1.2〜2.5 times that in oil-treated controls, respectively ; (b) an electron microscopic observation indicated EDC treatment to bring about apoptosis of lymphocytes which were embraced by stromal cells (i.e. macrophages) ; (c) flow cytometric analysis demonstrated EDC to induce the change of lymphocyte subsets : an increase in the suppressor/cytotoxic (CD4-CD8+) cells with decrease in the helper/inducer (CD4+CD8-) cells in Peyer s patch.
It follows from the above findings that EDC may cause morphologic changes in the murine immune organs closely related to T cell function. In addition, these changes appear to derive mainly from EDC-induced tissuespeciic gene expression. The results of the present study suggest that we must recognize the possibility that a lowdose EDC can affect the various immune responses in the capacity of immune disruptors.
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