DIRECT VISUALIZATION OF CALCIUM REFILLING INTO THE ENDOPLASMIC RETICULUM
Project/Area Number |
11670033
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General physiology
|
Research Institution | GUNMA UNIVERSITY |
Principal Investigator |
MOGAMI Hideo GUNMA UNIVERSITY INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION ASSISTANT PROFESSOR, 生体調節研究所, 助手 (90311604)
|
Project Period (FY) |
1999 – 2000
|
Project Status |
Completed (Fiscal Year 2000)
|
Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
|
Keywords | endoplasmic reticulum / calcium / Ca^<2+> ATP ase (calcium pump) |
Research Abstract |
The calcium binding capacity was about 1500〜2000 for the [Ca^<2+>]_c range of 150〜500 mM.The calcium binding capacity did not vary significantly with [Ca^<2+>]_c in this range. The calcium binding capacity of the ER us approximately 20. In pancreatic acinar cells we have therefore found a difference of two orders of magnitude in the efficiency of calcium buffering in the cytosol and the ER lumen. Active mitochondria, strategically placed around the secretory pole in pancreatic acinar cells, block Ca^<2+> diffusion from the primary Ca^<2+> release sites in the granule-rich area in the apical pole to the basal part of the cell containing the nucleus. When mitochondrial function is inhibited, this barrier disappears and the Ca^<2+> signals spread all over the cytosol.
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Report
(3 results)
Research Products
(9 results)