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The role of phosphatidylinositol turnover in nuclei

Research Project

Project/Area Number 11670138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

FUKAMI Kiyoko  Inst.Med.Sci., Dep.Biochem., The University of Tokyo, Associate Professer, 医科学研究所, 講師 (40181242)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPhospholipase C / Nuclei / Fertilization / Acrosome reaction
Research Abstract

We report that male mice in which the phospholipase C (PLC) δ4 gene was disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4^<-/-> sperm resulted in significantly fewer eggs becoming activated and, more importantly, the calcium transients associated with fertilization were absent or extremely delayed. Subsequent experiments demonstrated that PLCδ4^<-/-> sperm were unable to initiate/complete the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data show for the first time a physiological function of a PLCδ type, and demonstrate that PLCδ4 plays a significant role in the zona pellucida-induced acrosome reaction during mammalian fertilization.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kiyoko Fukami et al.: "Growthfactor-induced promoter activation of murine phospholipase C delta 4."Eur.J.Biochem.. 267. 28-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Ijuin et al.: "Identification and characterization of a novel inositol polyphosphate 5-phosphatase"J.Biol.Chem.. 275. 10870-10875 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Thorsten Laux et al.: "GAP43, MARCKS, and CAP23 modulate PI(4,5)P2 at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism."J.Cell Biol.. 149. 1455-1472 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hua Wu et al.: "Sperm factor induced [Ca2+]i oscillations by stimulating the phosphoinositide pathway."Biol.Reprod.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maiko Fukuoka et al.: "A novel N-WASP binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42."J.Cell Biol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koji, Nagano, Kiyoko Fukami, Tetsuya, Minagawa, Yutaka, Watanabe, Choichiro, Ozaki, and Tadaomi Takenawa: "A novel phospholipase Cδ4 splice variant as a negative regulator of PLC."J.Biol.Chem.. 274. 2872-2879 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tadaomi Takenawa, Toshiki Itoh, and Kiyoko Fukami: "Regulation of phosphatidylinositol 4, 5-bisphosphate levels and its roles in cytoskeltal re-organization and malignant transformation."Chemistry and Physics of Lipids. 98. 13-22 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Makiko Otsuki, Kiyoko Fukami, Takashi Kohno, Jun Yokota, and Tadaomi Takenawa: "Identification and characterization of a new phospholipase C-like protein, PLC-L2."Biochem.Biophys.Res.Comm.. 266. 97-103 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyoko Fukami, Kei Takenaka, Koji, Nagano, and Tadaomi Takenawa: "Growthfactor-induced promoter activation of murine phospholipase C delta 4."Eur.J.Biochem.. 267. 28-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takashi Ijuin, Yasuhiro Mochizuki, Kiyoko Fukami, Funaki, M Asano, T and Tadaomi Takenawa: "Identification and characterization of a novel inositol polyphosphate 5-phosphatase."J.Biol.Chem.. 275. 10870-10875 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Thorsten Laux, Kyioto Fukami, Marcus Thelen, Tamara Golub, Dunja Frey, and Pico Caroni: "GAP43, MARCKS, and CAP23 modulate PI (4, 5) P2 at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism."J.Cell Biol.. 149. 1455-1472 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maiko Fukuoka, Shiro Suetsugu, Hiroaki Miki, Kiyoko Fukami, Takeshi Endo, and Tadaomi Takenawa: "A novel N-WASP binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42."J.Cell Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyoko Fukami et al.: "Growthfactor-induced promoter activation of murine phospholipase C delta 4."Eur.J.Biochem.. 267. 28-36 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi Ijuin et al.: "Identification and characterization of a novel inositol polyphosphate 5-phosphatase"J.Biol.Chem.. 275. 10870-10875 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Thorsten Laux et al.: "GAP43, MARCKS, and CAP23 modulate PI(4,5)P2 at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism."J.Cell Biol.. 149. 1455-1472 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hua Wu et al.: "Sperm factor induced [Ca2+]i oscillations by stimulating the phosphoinositide pathway."Biol.Reprod.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Maiko Fukuoka et al.: "A novel N-WASP binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42."J.Cell Biol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] K. Nagano et al.: "A novel phospholipase Cδ4 splice variant as a negative regulator of PLC"J. Biol. Chem.. 274. 2872-2879 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Takenawa et al.: "Regulation of phosphatidylinositol 4,5-bisphosphate levels and its roles in cytoskeltal re-organization and malignant transformation"Chemistry and Physics of Lipids. 98. 13-22 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Imai et al.: "The pleckstrin homology domatin of the Wiskott-aldrich syndrome protein is involved in the organization of actin cytoskeleton"Clinical Immunology. 92. 128-137 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Otsuki et al.: "Identification and characterization of a new phospholipase C-like protein, PLC-L2"Biochem. Biophys. Res. Comm.. 266. 97-103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Fukami et al.: "Growthfactor-induced promoter activation of murine phospholipase C delta 4"Eur. J. Biochem.. 267. 28-36 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Ijuin et al.: "Identification and characterization of a novel inositol polyphosphate 5-phosphatase"J. Biol. Chem.. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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