| Project/Area Number |
11670140
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
OJI Yusuke Osaka University Medical School, Associate Professor, 医学部, 助手 (20294100)
|
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Haruo Osaka University Medical School, Professor, 医学部, 教授 (70162906)
SOMA Toshihiro Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助手 (40273619)
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | WT1 gene / Lung cancer / ウイルムス腫瘍遺伝子 / WT1 |
|---|
| Research Abstract |
We have previously demonstrated that wild-type Wilms' tumor gene (WT1) is expressed at high levels in both leukemic blast cells and various types of solid tumor cell lines and the growth of these cells was inhibited by the treatment with WT1 antisense oligomers. In the present project, we examined WT1 gene expression in fresh lung cancer tissues and in matched normal lung tissues from 20 patients with lung cancer (14 with adenocaricinoma, 6 with squamous-cell carcinoma) by Real time RT-PCR method. We demonstrated that the WT1 gene was expressed in most of lung cancer tissues examined. The levels of WT1 expression in lung cancer tissues were 10-3000 times higher than that in normal lung tissues. Furthermore, we identified WT1 gene product as a novel tumor antigen of leukemia. These results suggest that oncogenic role of WT1 in tumorigenesis of lung cancer and the possibility that WT1 protein could serve as a target protein for tumor-specific immunity.
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