| Project/Area Number |
11670147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
NAKAMURA Mitsuru Jichi Med.Sch., Med., Lecturer, 医学部, 講師 (20198237)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | E-Selectin / Sialyl-LeX / Counter Ligand / Cell Adhesion / Sialomucin Family / O-Linked Glycoprotein / Sialyl-LeX / Sialomucin Family / O-linked glycoprotein |
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| Research Abstract |
In human pre-B leukemia cells sialyl-LeX (sLeX) antigen is not expressed on every protein but expressed on a specific core glycoprotein with molecular size of 150kDa (gp150). In addition, cell surface sLeX antigen expression level is controlled by core 2 N-acetylglucosaminyltransferase (C2GnT) gene. In addition, we have revealed the followings.
1. Not only in pre-B cell differentiation but also in tonsillar B cell activation, C2GnT regulates cell surface sLeX expression level.
2. Anti-sLeX mAb reactivity of gp150 was disappeared after O-sialoglycoprotein-endopeptidase (from Pasteurella haemolytica) treatment. This suggests gp150 must be a sialomucin type glycoprotein molecule that has abundant O-linked sugar chains with sialic acid on their termini.
3. A tryptic peptide was purified and analyzed from pre-B NALL-1 cell line membranous fraction using anti-sLeX mAb-conjugated protein-G.The peptide was determined as a mucin-type glycoprotein. It was confirmed by immunostaining methods.
4. The cDNA of gp150 was obtained and transfected in the antisense orientation into a sLeX-highly expressed pre-B cell line. Sialyl-LeX-reactivity in the transfected cell line was significantly downregulated and E-selectin-dependent cell adhesion capability was remarkably reduced.
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