| Project/Area Number |
11670153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
KANNO Hitoshi Nihon University, School of Medicine, Assistant Professor, 医学部, 講師 (70221207)
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| Co-Investigator(Kenkyū-buntansha) |
MIWA Shiro Okinaka Memorial Institute for Medical Research, Professor Emeritus, 顧問研究員 (40034954)
AIZAWA Shin Nihon University, School of Medicine, Associate Professor, 医学部, 助教授 (30202443)
NAKAGAWA Shigeki Nihon University, School of Medicine, Professor, 医学部, 教授 (40059465)
OYAIZU Naoki Tokyo Medical and Denatal University, Dept.Retroviral Regulation, Associate Professor, 医学部, 助教授 (00282773)
KOIZUMI Tsutomu Fukui Medical University, Experimental Animal Center, Associate Professor, 動物実験施設, 助教授 (40126579)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | hemolytic anemia / apoptosis / glycolysis / reactive oxygen species / disease model animals / Friend cells / 遺伝子治療 / トランスジェニックマウス |
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| Research Abstract |
Pyruvate kinase (PK) is a rate-limiting enzyme of the glycolytic pathway and PK deficiency is one of the most prevalent causes of hereditary non-spherocytic hemolytic anemia. The Pk-1^<slc> mouse is found to be a spontaneous mutant of the murine LR-type PK gene, and manifests hemolytic anemia and splenomegaly. We showed that apoptotic cells were notably increased in spleen of the Pk-1^<slc> by the TUNEL method, and that the erythroid-specific expression of normal PK gene ameliorated apoptosis in the genetic rescue experiment. Flow cytometric analysis showed that Annexin V / Ter119-double positive cells were significantly increased in the Pk-1slc mouse compared to wild type CBA mice, confirming apoptotic cells were of erythroid lineage. In this study, we examined a physiological role of glycolysis upon apoptosis of the erythroid cells using two Friend erythroleukemic cells, SLC and CBA.These cell lines have been establushed from the Pk-1^<slc> and a wild-type CBA mouse, respectively. As previously reported, apoptosis spontaneously occurred in the Friend cells and was inducible in both cells by glucose deprivation or 0.1-20mM 2-deoxyglucose (2DG). SLC cells were more susceptible to apoptosis by either shorter exposure to glucose deprivation or lower 2DG concentration. The detailed mechanism are still unclear, but we have shown that reactive oxygen species (ROS) might be accountable for these apoptotic changes.
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