| Project/Area Number |
11670180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KON Shinichiro Sapporo Medical University School of Medicine, AssociateProfessor, 医学部, 助教授 (10195886)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Kiyoshi Sapporo Medical University School of Health Sciences, Professor, 保健医療学部, 教授 (70175272)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2000: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | MALT lymphoma / immunoglobulin gene / somatic mutation / clonality / Southern blot analysis / 悪性リンパ腫 / MALTリンパ腫 / 遺伝子再構成 / サザンブロット / PCR / somatic mutation |
|---|
| Research Abstract |
Tissues from lymphoproliferative disorders arising in mucosa associated lymphoid tissue were analysed histopathologically, immunohistochemically and also by molecular techniques. Significant number of the cases were diagnosed as malignant lymphomas by demonstrating their monoclonality by Southern blot analysis using immunoglobulin gene probes. Biased usage of D segment of complementarity determining region 3 (CDR3) was obserbed in cases of ocular adnexal lymphoma, suggesting specific antigenic atimuli for generating malignant lymphomas in this region. Identification of VDJ construction was useful to know clonal expansion in separate regions and analysis of somatic mutations in variable region was also useful to recognize clonal development in multiple regions.
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