| Project/Area Number |
11670181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
IKEDA Tatsuru Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 講師 (40202890)
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| Co-Investigator(Kenkyū-buntansha) |
CHIBA Hideki Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 講師 (00295346)
SAWADA Norimasa Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (30154149)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | pericyte / osteoblast / smooth-msucle actin / differentiation / 分化機能 |
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| Research Abstract |
1. The human osteoblastic cell line, SV-HFO, expressed the pericyte markers including a-smooth muscle actin (SMA), calponin and 3G5. The expression of them was constantly mainteined during osteoblastic differentiation induced by dexamethasone. In addition, the pattern of expression of SMA was influenced by TGF-betal and retinoic acid. Both TGF-betal and retinoic acid decreased the number of SMA positive cells. Interestingly, TGF-betal transiently incereased the number of SMA positive cells in the very early stage of osteoblastic differentiation (the 4 day). Since TGF-beta and retinoic acid are the inhibitors of the mineralization of SV-HFO, the inhibitory effect of them on SMA expression suggested that osteoblastic differentiation and A pericyte differentiation are regulated by the same molecular and biochemical mechanism. SV-HFO W cells share many characteristics with pericytes. Furthermore, many studies have also suggested pericytes are the precursors to osteoblasts. Hence, we postulate that SV-HFO cells are possibly identical to pericytes. Whether SV-HFO cells function as pericytes during angiogenesis in vitro or in vivo warrant further investigation.
2 .We investigated the expression of monoclonal antibody 3G5, which recognizes a ganglioside present on the surface of pericytes, on brain capillaries in situ. The antibody mainly showed immunoreactivity with pericytes located at the branching point or the curved area of the capillaries. Double staining for calponin or SMA and 3G5 demonstrated that pericytes of mid-capillaries expressed both calponin and 3G5, but not SMA. Since antibody 3G5 is not available for the paraffin sections, calponin should be a better pericyte marker than SMA or 3G5 and useful for immunohistochemical study to investigate tumor angiogenesis.
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