| Project/Area Number |
11670190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
TAKANO Yasuo TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, Medicine, Pathology, professor chairman, 医学部, 教授 (60142022)
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| Co-Investigator(Kenkyū-buntansha) |
MURAI Yoshihiro TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, Medicine, Pathology, Research Associate, 医学部, 助手 (60115194)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Survivin / Y-49 / malognantlymphoma / Gastric cancer / malignant lymphoma / survivin / gastric cancer / survivin associated antigen |
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| Research Abstract |
We have performed these subjects as below during the term of Grant-in-Aid for Scientific Research (C).
1) We have produced a monoclonal antibody, named as Y-49, against synthesized polypeptides selected from survivin amino-acid sequence. This antibody did not react survivin but recognized some antigen reacting to proliferating cells and malignant cells in the immunohistochemical study. Three bands were noticed at 22, 60 and 85 kds on the basis of Western blotting of cultured lymphoma cells.
2) After twice of subcloning, 22 kd band became dense and clear, and cell fractionation revealed that only 22 kd protein existed in the nuclei of lymphoma cells and those three proteins were present in the cytoplasm.
3) Flow cytometric analysis using PHA stimulated normal lymphocytes disclosed that Y-49 was strongly labeled in the cell cycle of S and G2/M.
4) An immunohistochemical study for malignant lymphoma cases employing Y-49 resulted : (1) Y-49 was highly labeled in extranodal and diffuse large cell lymphomas.(2) High labeling indices of Y-49 represented worse prognosis of lymphoma patients with statistics.(3) By multivariate analysis, high labeling indices of Y-49 was an independent prognostic factor.
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