| Project/Area Number |
11670215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tottori University |
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Principal Investigator |
KITAMURA Yukisato Tottori University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20204919)
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| Co-Investigator(Kenkyū-buntansha) |
NANBA Eiji Tottori University, Gene Research Center, Associate Professor, 遺伝子実験施設, 助教授 (40237631)
TERADA Tadashi Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (30188677)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | hepatic stellate cell / simian virus 40 / temperature-sensitive / cell line / growth / differentiation |
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| Research Abstract |
Hepatic stellate cell (HSC) is one type of perisinusoidal cells in the liver and has many unique characteristics. HSC produce various cytokines and growth factors, and play an important role on the various pathophysiological state of liver diseases. Investigators carried out this project for studying the gene mechanism of HSC growth and differentiation, and for gene cloning of new factors which play an important role on HSC growth and differentiation.
Investigators selected and cloned a specific gene which expressed under two conditions, the first was that HSC expressed large T antigen (T-ag) and the second was that HSC did not express T-ag, using temperature-sensitive mutant of simian virus 40. The specific genes which were thought to express in an active phenotype of HSC and in a quiescent phenotype of HSC were sequenced. It was suggested that these genes played an important role on the pathophysiological state of chronic hepatitis and liver cirrhosis in human liver tissue. Investigators also studied the signal transduction in HSC about fibrogenesis and this study is now being submitted.
This project is now carrying out for an antibody production of new factors from HSC and for gene analysis. It is the final aim that the gene therapy against fibrosis is established.
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