| Project/Area Number |
11670249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Keio University |
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Principal Investigator |
TANABE Masanobu Keio University Dep't of Trop.Med.& Parasitol.Assistant Professor, 医学部, 専任講師 (80051928)
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| Co-Investigator(Kenkyū-buntansha) |
OKURA Tamiko Keio University Inst.Adv.Med.Res.Instructor, 医学部, 助手 (20051740)
YAMADA Taketo Keio University Dep't of Pathology Assistant Professor, 医学部, 専任講師 (60230463)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Schistosoma mansoni / Egg granuloma / Convulsion-inducing factor / Monoclonal antibody / Lipoprotein / Apoprotein |
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| Research Abstract |
A convulsion-inducing lipoprotein (CILIP) was purified from hepatic egg granulomas and spleens of mice infected with Schistosoma mansoni, and its physical and chemical properties were characterized. The CILIP-like convulsion-inducing substances could be also purified from various tissues of uninfected and infected mice with S.mansoni, several inbred strains and immunocompromised mice cared under SPF condition, rat, rabbit, and human according to the method developed for CILIP purification. These substances showed the same physical and chemical properties as CILIP.Intravenous injection of these substances caused the same sign and symptoms as have been demonstrated by CILIP injection. Moreover, in vivo study indicates that these substances may act through the same mechanism as CILIP.These substances are, therefore, considered to be compatible lipoproteins with CILIP.It is, therefore, likely that CILIP may be widely distributed in various animal and human tissues.
Rabbit and mouse polyclonal antibody to CILIP completely inhibited not only CILIP activity but also the activities of convulsion-inducing substances isolated from various animal and human tissues. This evidence suggests that there are a common molecule among these convulsion-inducing lipoproteins. The existence of two types of common molecules in the convulsion-inducing lipoproteins isolated from various animal and human tissues was confirmed by two dimensional electrophoresis. All of these evidences suggest that two types of common peptides probably play an essential role in the pharmacological action of CILIP.
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