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Development of assays detecting antibodies to parasite proteinases for immunodiagnosis of parasitic diseases

Research Project

Project/Area Number 11670255
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionKanazawa Medical University

Principal Investigator

IKEDA Teruaki  Kanazawa Medical University, Medical Zoology, Associate Professor, 医学部, 助教授 (70064607)

Co-Investigator(Kenkyū-buntansha) OIKAWA Yosaburo  Kanazawa Medical University, Medical Zoology, Research Assistant, 医学部, 助手 (10139785)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsparagonimiasis / fascioliasis / immunodiagnosis / cysteine proteinase / cystatin / Protein A / IgE / immunocapture / プロテインA / 蛍光基質
Research Abstract

Enzyme-linked immunosorbent assays (ELISAs) which detect specific antibodies to fluke cysteine proteinases have provided good sensitivity and specificity for immunodiagnosis of trematode diseases. To detect the specific antibodies without the need for purified proteinase antigens, three immunocapture assays using cystatin (protein inhibitor of cysteine proteinase), Protein A, or anti-human IgE monoclonal antibody were applied for the immunodiagnosis of paragonimiasis and fascioliasis. In a cystatin capture ELISA, cystatin-sensitized plate wells were incubated with a preparation containing fluke cysteine proteinases and with diluted patient sera, and standard ELISA procedures were then followed. In Protein A and anti human IgE antibody capture assays, sensitized wells were incubated with diluted patient sera, the fluke proteinase preparation, then fluorogenic peptidyl substrate for fluke cysteine proteinases. These two assays detected specific IgG and IgE, respectively, to fluke cysteine proteinases by measuring proteinase activity on the plate wells. The three immunocapture assays, which detected IgG and IgE specific for fluke cysteine proteinases, provided a high sensitivity and specificity for immunodiagnosis of paragonimiasis and fascioliasis. The detection of specific IgE is superior to that of specific IgG in evaluating the efficacy of chemotherapy.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 池田照明: "Capture ELISAとCapture蛍光法による新しい寄生虫症免疫診断法"医学のあゆみ. 194(3). 156-157 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Ikeda: "Effect of ionophores on in vitro excystment of Paragoniwus ohiral metacercariae"Parasitol.Res.. 87(4). 343-344 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Ikeda: "I Protein A immunocapture assay detecting antibodies to fluke cysteine proteinases for imuiunodiagnosis of human paragonimiasis and fascioliasis"J.Helminthol. 75(3). 245-249 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Ikeda: "Perspectives of helminthology (eds.N.Chowdhury, I.Tada), Immunodiagnosis of Helminthic Diseases"Science Publishers.USA. 531(397-418) (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda,T.: "Effect of ionophores on in vitro excystment of Paragonimus ohirai metacercariae"Parasitol. Res.. 87 (4). 343-344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda,T.: "Protein A immunocapture assay detecting antibodies to fluke cysteine proteinases for immunodiagnosis of human paragonimiasis and fascioliasis"J Helminthol.. 75 )3). 245-249 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda,T.: "Immunodiagnosis of Helminthic Diseases"Perspectives of helminthology, )eds. Chowdhury,N., Tada,I.). 397-418 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 池田照明: "Capture ELISAとCapture蛍光法による新しい寄生虫症免疫診断法"医学のあゆみ. 194. 156-157 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikeda T.: "Effect of ionophores on in vitro excystment of Paragonimus ohirai metacercariae."Parasitol.Res.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikeda T.: "Protein A immunocapture assay detecting antibodies to fluke cysteine proteinases for immunodiagnosis of human paragonimiasis and fascioliasis."J.Helminthol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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