| Project/Area Number |
11670260
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE |
|---|
Principal Investigator |
NAGATA Toshi HAMAMATSU UNIV SCH MED, ASSOCIATE PROFESSOR, 医学部, 助教授 (90275024)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Masato UNIV OF RYUKYUS SCH MED, RESEARCH ASSOCIATE, 医学部, 助手 (10242778)
UCHIJIMA Masato HAMAMATSU UNIV SCH MED, RESEARCH ASSOCIATE, 医学部, 助手 (20252174)
KOIDE Yukio HAMAMATSU UNIV SCH MED, FULL PROFESSOR, 医学部, 教授 (30126809)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | DNA VACCINE / DELAYED-TYPE CELLULAR IMMUNITY / LISTERIA / INVARIANT CHAIN / MHC CLASS II BINDING PEPTIDE / T CELL EPITOPE |
|---|
| Research Abstract |
Listeria epitope-specific helper T cells (Th) were successfully primed in vivo by DNA immunization with a plasmid carrying class II-associated invariant chain (Ii) peptide (CLIP)-replaced murine Ii molecule. Immunizations by gene gun bombardment with plasmids carrying Ii molecules, whose CLIP regions were replaced with a Listeria-specific Th epitope, listeriolysin O (LLO) 215-226 (H2-E^k-restricted) or p60 301-312 (H2-A^d-restricted), was capable of priming specific Th showing proliferative responses to respective peptides in both C3H/He (H-2^k) and BALB/c (H-2^d) mice. Although the immunization to C3H/He mice induced specific interferon-γ-producing splenocytes and conferred significant protective immunity against listerial infection, the immunized BALB/c mice failed to do so. These results indicate that our CLIP-replaced Ii expression DNA vaccines are able to prime specific Th with different effector functions.
|
