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Mechanisms of inhibition of drug-induced ulcer formation by intestinal bacteria

Research Project

Project/Area Number 11670265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionThe University of Tokushima, School of Medicine

Principal Investigator

KEIKO Kataoka  The University of Tokushima, School of Medicine, Department of Bacteriology, Assistant, 医学部, 助手 (40189303)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Haruyuki  The University of Tokushima, School of Medicine, Department of Bacteriology, Assistant, 医学部, 助手 (80294669)
OHNISHI Yoshinari  The University of Tokushima, School of Medicine, Department of Bacteriology, Professor, 医学部, 教授 (10037400)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsnonsteroidal antiinflammatory drug / small intestinal ulcer / Gram-negative rods / Lactobacillus acidophilus / antioxidants / bile / transit index / diclofenac / 脂質過酸化 / アスコルビン酸 / Escherichia coli
Research Abstract

(1) Nonsteroidal antiinflammatory drug 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (BFMeT)-induced ulcer formation in the small intestine of rats was repressed by giving the animals a culture supernatant of Lactobacillus acidophilus as drinking water. The supernatant had antioxidative activity and repressed the increase of percentage of Gram-negative rods in the ileal contents and the accumulation of thiobarbituric acid (TBA)-reactive substances in the ileal mucosa. Among tested antioxidants, ascorbic acid most effectively repressed BFMeT-induced ulcer formation.
(2) The number of BFMeT-induced ulcers was decreased in the common bile duct-cecum cannulated rats, in which bile was not secreted into the small intestine. However, the percentage of Gram-negative rods did not increase without BFMeT in the rats. BFMeT did not increase the bile volume secreted for 24 hr after BFMeT treatment. Transit index, a marker of gastrointestinal motility, was not significantly different between the gum arabic-treated group and the BFMeT-teated group until 72 hr after BFMeT treatment. Ileal contents of rats treated with BFMeT or gum arabic had no effect on viable cell number of Escherichia coli and Lactobacillus acidophilus when these bacteria were incubated in the supernatants of the contents.
(3) Diclofenac dose-dependently induced ulcer formation in the small intestine and increased the percentage of Gram-negaive rods in the ileal contents.
(4) A hypolipidemic drug, fluvastatin, that has antioxidative activity repressed BFMeT-induced ulcer formation in the small intestine of rats.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Arimochi,H. et al.: "Effects of β-glucuronidase-deficient and lycopene-producing Escherichia coli strains on formation of Azoxymethane-induced aberrant crypt foci in the rat colon."Biochemcal Biophyscal Research Communication. 262. 322-327 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chewonarin,T. et al.: "Effects of roselle (Hibiscus sabdariffa Linn.), a Thai medicinal plant, on the mutagenicity of various known mutagens in Salmonella typhimurium and on formationof aberrant crypt foci induced by the colon carcinogens azoxymethane and 2-amino-1-methyl-6-phenylimidazo 〔4, 5-bjpyridine in F344 rats."Food and Chemical Toxicology. 37. 591-601 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chiampanichayakul,S. et al.: "Inhibitory effects of bitter melon (Momordica charantia Linn.) on bacterial mutagenesis and aberrant crypt focus formation in the rat colon."Journal of Medical Investigation. 48(1/2)(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuwahara,T. et al.: "Genetic variation in 16S-23S rRNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species."Microbiology and Immunology. 45(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuwahara,T. et al.: "PCR-dot blot hybridization based on the neuraminidase-encoding gene is useful for detection of Bacteroides fragilis."Microbiology and Immunology. 45(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arimochi, H.et al.: "Effects of β-glucuronidase-deficient and lycopene-producing Escherichia coli strains on formation of azoxymethane-induced aberrant crypt foci in the rat colon."Biochemcal Biophyscal Research Communication. 262. 322-327 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chewonarin, T.et al.: "Effects of roselle (Hibiscus sabdariffa Linn.), a Thai medicinal plant, on the mutagenicity of various known mutagens in Salmonella typhimurium and on formation of aberrant crypt foci induced by the colon carcinogens azoxymethane and 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine in F344 rats."Food and Chemical Toxicology. 37. 591-601 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chiampanichayakul, S.et al.: "Inhibitory effects of bitter melon (Momordica charantia Linn.) on bacterial mutagenesis and aberrant crypt focus formation in the rat colon."Journal of Medical Investigation. 48(1/2)(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuwahara, T.et al.: "Genetic variation in 16S-23S rRNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species."Microbiology and Immunology. 45 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuwahara, T.et al.: "PCR-dot blot hybridization based on the neuraminidase-encoding gene is useful for detection of Bacteroides fragilis"Journal of Medical Investigation. 48 (1/2)(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chiampanichayakul,S. et al.: "Inhibitory effects of bitter melon (Momordica charantia Linn.) on bacterial mutagenesis and aberrant crypt focus formation in the rat colon."Journal of Medical Investigation. 48(1/2)(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuwahara,T. et al.: "Genetic variation in 16S-23S rRNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species."Microbiology and Immunology. 45(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuwahara,T. et al.: "PCR-dot blot hybridization based on the neuraminidase-encoding gene is useful for detection of Bacteroides fragilis."Journal of Medical Investigation. 48(1/2)(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Arimochi,h.et al.: "Effects of β-glucuronidase-deficient and iycopene-producing Escherichia coli strains on formation of azoxymethane-induced aberrant crypt foci in the rat colon."Biochem.Biophys.Res.Commun.. 262. 322-327 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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