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AN IDENTIFICATION AND CHARACTERIZATION OF MENBRANE PROTEIN ASSOCIATED WITH CELL ACTIVATIONS LY BACTERIAL LIPOPOLYSACCHARIDES

Research Project

Project/Area Number 11670270
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionINTERNATIONAL MEDICAL CENTER OF JAPAN

Principal Investigator

KIRIKAE Teruo  RESEARCH INSTITUTE NTERNATIONAL MEDICAL CENTER OF JAPAN, INFECTIOUS DISEASE AND TROPICAL MEDICINE, DIRECTOR, 感染・熱帯病研究部, 部長 (50192563)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsLPS / LIPOPOLYSACCHARIDE / ENDOTOXIN / LIPIDA / MACROPHAGE / PE-406 / PE-506 / STOROMA-DERIVED CELL / Lipoplysaccharide / LPS結合タンパク質 / タキソール
Research Abstract

A bacterial endotoxin lipopolysaccharide (LPS) is known to induce the expression of various cytokines via activation of host cells, including macrophages. To identify and characterize the novel molecules which play integral roles in the LPS-induced cell activation, we utilized antibodies against membrane proteins and isotopically labeled lipid A, and detected some candidates. We have previously shown that murine stroma-derived ST2 cells do not express CD14, and have approximately the same LPS responsiveness as macrophages. We here raised polyclonal antibodies against purified cell membrane from ST2 cells, as well as LPS binding protein partially purified from the ST2 cell membrane fraction by HPLC.Expression of particular proteins in the macrophage membrane was observed by concentration, separation and visualization of macrophage membrane proteins by immunoprecipitation and subsequent Western blot with antibodies raised against the membrane fraction. We also detected 57 kDa and 53kDa proteins as lipid A binding proteins by ligand blot, respectively with [^3H]-lipid A precursor (PE-406) and [^3H]-lipid A (PE-506).

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Kirikae,T.,Kirikae,F.,Iwai,H.,Qureshi,N.,Fukase,K., et al.: "LPS-dependent changes in the expression of 57 kDa and 53 kDa cell membrane proteins without participation of CD14."J.Endotoxin Res.. 5. 62-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakano,M.,Tominaga,K.,Saito,S.,Kirikae,F,,Lin,S, et al.: "Lipopolysaccharide- and paclitaxel (Taxol)- induced tolerance in murine peritoneal macrophages."J.Endotoxin,Res.. 5. 102-106 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirakata,Y.,Kirikae,T.,Kirikae,F.,Yamaguchi,T., et al.: "Effect of Psudomonas aeruginosa exotoxin A on endotoxin-induced tumor necrosis factor production in murine lung."J.Med.Microbiol.. 48. 471-477 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae,T.,Nitta,T.,Kirikae,F.,Suda,Y., et al.: "Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a different way from LPS of Salmonella."Infect.Immun.. 67. 1736-1742 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito,S.,Matuura,M.,Tominaga,K.,Kirikae,T., et al.: "Important role of membrane-associated CD14 in the induction of IFN-g and subsequent nitric oxide production by murine macrophages in response to bacterial lipopolysaccharide."Eur.J.Biochem.. 267. 37-45 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Swierzko,,A.S.,Kirikae,T.,Kirikae,F.,Hirata,M., et al.: "Biological activities of Proteus lipopolysaccharides and their interaction with polymyxin B and a 18-kilodalton cationic antimicrobial protein (CAP18)-derived peptide."J.Med.Microbiol.. 49. 127-138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akashi,S.,Ogata,H.,Kirikae,F.,Kirikae,T., et al.: "Regulatory roles for CD14 and phosphatidylinositol in the signaling via Toll-like receptor 4-MD-2."Biochem.Biophys.Res.Commun.. 268. 172-177 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae,T.,Ojima,I.,Fuero-Oderda,C.,Lin,S., et al.: "Structural significance of the acyl group at the C-10 position and the A ring of the taxane core of paclitaxel for inducing nitric oxide and tumor necrosis factor production by murine macrophages."FEBS Lett.. 478. 221-226 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae,T.,Suda,Y.,Tamura,H.,Yamasu,H.,Kirikae,F., et al.: "Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis."Jpn.J.Infect.Dis.. 53. 34 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohmura,Y.,Kirikae,T.,Kirikae,F.,Nakano,M.,Sato,I. et al.: "Onapristone (ZK299) blocks the suppresive effect of progesterone, but not that of dexamethasone, on inducible nitric oxide synthase gene expression and nitric oxide production in murine macrophages."Int.J.Immunopharmaco.. 22. 765-774 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawano,C.,Muroi,K.,Yoshizaka,M.,Kirikae,T.,Ozawa,K., et al.: "Filamented Pseudomonas aeruginosa and a markedly high level of endotoxin in cerebrospinal fluid."J.Infect.. 41. 114-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohmura,Y.,Kirikae,T.,Kirikae,F.,Nakano,M.,Sato,I et al.: "Lipopolysaccharide (LPS)-induced intra-uterine fetal death (IUFD) in mice is principally due to maternal cause but not fetal sensitivity to LPS."Microbiol.Immunol.. 44. 7897-7904 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kakinuma,N.,Iwai,H.,Takahashi,S.,Hamano,K., et al.: "Quinolactacins A, B and C: novel quinolone compounds from Penicillium sp.EPF-6."J.Antibiot. 53. 1252-1256 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto,M.,Kirikae,F.,Dohi,T.,Kusumoto,S., et al.: "Structural elucidation of capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from patient with Crohn's disease."Eur.J.Biochem.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto,M.,Kirikae,F.Toyooka,K.,Kaneko,A., et al.: "Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis."Microbiol.Immunol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae, T., Kirikae, F., Iwai, H., Qureshi, N., Fukase, K., et al: "LPS-dependent changes in the expression of 57 kDa and 53 kDa cell membrane proteins without participation of CD14."J.Endotoxin Res. 5. 62-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] "Lipopolysaccharide- and paclitaxel (Taxol)- induced tolerance in murine peritoneal macrophages."J.Endotoxin, Res.. 5. 102-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirakata, Y., Kirikae, T., Kirikae, F., Yamaguchi, T., et al: "Effect of Psudomonas aeruginosa exotoxin A on endotoxin-induced tumor necrosis factor production in murine lung"J.Med. Microbiol.. 48. 471-477 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] "Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/Hel macrophages in a different way from LPS of Salmonella"Infect. Immun.. 67. 1736-1742 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] "Important role of membrane-associated CD14 in the induction of IFN-g and subsequent nitric oxide production by murine macrophages in response to bacterial lipopolysaccharide."Eur. J.Biochem.. 267. 37-45 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Swierzko, A.S., Kirikae, T., kirikae, F., Hirata, M., et al.: "Biological activities of Proteus lipopolysaccharides and their interaction with polymyxin B and a 18-kilodalton cationic antimicrobial protein (CAP18) -derived peptide."J.Med. Microbiol.. 49. 127-138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] "Regulatory roles for CD14 and phosphatidylinositol in the signaling via Toll-like receptor 4-MD-2."Biochem. Biophys. Res. Commun.. 268. 172-177 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae, T., Ojima, I., Fuero-Oderda, C., Lin, S., et al.: "Structural significance of the acyl group at the C-10 position and the A ring of the taxane core of paclitaxel for inducting nitric oxide and tumor necrosis factor production by murine macrophages."FEBS Lett.. 478. 221-226 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae, T., Suda, Y., Tamura, H., Yamasu, H., Kirikae, F., et al.: "Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis."Jpn. J.Infect. Dis. 53. 53-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohmura, Y., Kirikae, T., Kirikae, F., Nakano, M., Sato, I., et al: "Onapristone (ZK299) blocks the suppresive effect of progesterone, but not that of dexamethasone, on inducible nitric oxide synthase gene expression and nitric oxide production in murine macrophages."Int. J.Immunopharmaco.. 22. 765-774 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawano, C., Muroi, K., Yoshizaka, M., Kirikae, T., Ozawa, K., et al.: "Filamented Pseudomonas aeruginosa and a markedly high level of endotoxin in cerebrospinal fluid"J.Infect.. 41. 114-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohmura, Y., Kirikae, T., Kirikae, F., Nakano, M., Sato, I et al: "Lipopolysaccharide (LPS)-induced intra-uterine fetal death (IUFD) in mice is principally due to maternal cause but not fetal sensitivity to LPS."Microbiol. Immunol.. 44. 7897-7904 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kakinuma, N., Iwai, H., Takahashi, S., Hamano, K., et al.: "Quinolactacins A, B and C novel quinolone compounds from Penicillium sp EPF-6."J.Antibiot.. 53. 1252-1256 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto, M., Kirikae, F., Dohi, T., Kusamoto, S., et al.: "Structural elucidation of capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from patient with Crohn's disease."Eur. J.Biochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto, M., Kirikae, F., Toyooka, K., Kaneko, A., et al: "Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis."Microbiol. Immunol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kirikae,T.,Nitta,T.,Kirikae,F., et al.: "Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a different way from LPS of Salmonella."Infect.Immun. 67. 1736-1742 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saito,S.,Matuura,M.,Tominaga,K.,Kirikae,T., et al.: "Important role of membrane-associated CD14 in the induction of IFN-g and subsequent nitric oxide production by murine macrophages in response to bacterial lipopolysaccharide."Eur.J.Biochem.:,. 267. 37-45 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Swierzko,,A.S.,Kirikae,T.,Kirikae,F.,Hirata,M., et al.: "Biological activities of Proteus lipopolysaccharides and their interaction with polymyxin B and a 18-kilodalton cationic antimicrobial protein (CAP18)-derived peptide."J.Med.Microbiol.:. 49. 127-138 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akashi,S.,Ogata,H.,Kirikae,F.,Kirikae,T., et al.: "Regulatory roles for CD14 and phosphatidylinositol in the signaling via Toll-like receptor 4-MD-2."Biochem.Biophys.Res.Commun.. 268. 172-177 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashimoto,M.,Kirikae,F.Toyooka,K.,Kaneko,A., et al.: "Protective Effect of OK-432 on Mice against Endotoxemia and Infection with Pseudomonas aeruginosa and Salmonella enteritidis."Microbiol.Immunol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Teruo Kirikae: "Lipopolysaccharides (LPS) of oral Black-pigmented Bacteria induce Tumor Necrosis Factor Production by LPS-Refractory C3H/HCJ Macrophages in a way different"INFECTION AND IMMUNITY. Vol. 67 No. 4. 1736-1742 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Anna st Swierzko: "Biological activities of ipopolysaccharides of proteus spp and their in teraction with polymyxin B and 18-k Dacationic antimicrobial protein (CRP-18) derived peptide"J. Med. Microbiol.. 49. 127-138 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 切替照雄: "エンドトキシンと抗腫瘍薬パクリタキセル"MINOPHAGEN MEDICAL REVIEW. 44.6. 313-326 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Teruo Kirikae: "LPS-dependent changes in the expression of 57kDa and 53kDa cell membrane proteins without participation of C14"J. Endotoxin Res.. 5. 62-65 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masayasu Nakano: "Lipopolysaccharide- and paditaxel (Taxol) ?induced tolerance in murine peritoneal macrophages"J. Endotoxin Res.. 5. 102-106 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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