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The mechanism of acquisition of methicillin resistance

Research Project

Project/Area Number 11670272
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionJUNTENDO UNIVERSITY

Principal Investigator

ITO Teruyo  JUNTENDO UNIVERSITY, Lecturer, 医学部, 講師 (10095763)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsMRSA (methicillin-resistant Staphylococcus aureus) / mecA / PBP2 / SCCmec(staphylococcal cassette chromosome mec) / excision / integration / ccrA, B (cassette chromosome recombinase A, B) / mobile genetic element / ペニシリン結合蛋白2'(PBP2') / 転移 / ccrA / ccrB / メチシリン耐性
Research Abstract

(1) Cloning and determination of three types of SCCmec.
beta-lactam resistance gene mecA of Staphylococcus aureus is carried by a nova mobile genetic element, designated staphylococcal cassette chromosome mec(SCCmec)
We have cloned and determined the nucleotide sequence of SCCmecs of three strains, NCTC10442(isolated in England in 1961), N315(isolated in Japan in 1982), and 85/2082 (isolatd in New Zieland in 1985). There were substantial differences in the size and nucleotide sequences between the elements and the SCCmec. Structural analysis of the new elements revealed that they possessed all the salient features of the SCCmec ; conserved terminal inverted repeats and direct repeats at the integration junction points ; conserved genetic organization around mecA gene ; and the presence of cassette chromosome recombinase(ccr) genes responsible for the movement of SCCmec.
(2) Mobility of SCCmec.
We have identified two movel genes, ccrA and ccrB, encoding polypeptides having a partial homology to recombinases of the invertase/resolvase family. The open reading frames were found to catalyze precise excision of the SCCmec from the methicillin-resistant S.aureus chromosome and site-specific as well as orientation-specific integration of the SCCmec into the S.aureus chromosome when introduced into the cells as a recombinant multicopy plasmid.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] 伊藤輝代: "Cloning and nucleotide sequence determination of the entire mecDNA of pre-methicillin resistant staphylococcus auveus N315"Antimicrob.Agents and Chemother.. 43. 1449-1458 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 片山由紀: "A new class of genetic element, staphylococcal cassette chromosome mec, encodes methicillin resistance in staphylococcus auveus"Antimicrob.Agents and Chemother.. 44. 1549-1555 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "Complete structure of three types of staphylococcus cassette chromosome mec CSC(mec) integrated in the chromosome of MRSA strains in the world."Antimicrob.Agents and Chemother.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "メチシリン耐性の伝播に関与する mobile genetic element (staphylococcal cassette chromosome mec)"日本細菌学雑誌. 55. 483-498 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "S.anveusのvancomycinおよびmethicillin耐性"日本化学療法学会雑誌. 48. 7-23 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "疫学的解析と遺伝子検査-MRSAの分子疫学"臨床と細菌. 26. 177-182 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 片山由紀: "メチシリン耐性領域の正確な切り出しと部位特異的挿入"臨床と微生物. 26・2. 100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "知っておきたい現代感染症事情"医歯薬出版株式会社. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "臨床遺伝子医学ガイダンス"南山堂. 312 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "エマージングディジーズ"近代出版. 425 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "分子予防医学"医学書院. 384 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "バイオサイエンスの新世紀 第13巻 生体防禦"共立出版. 236 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ito, T.Y.Katayama, K.Hiramatsu: "Cloning and nucleotide sequence determination of the entire mecDNA of pre-methicillin-resistant Staphylococcus aureus N315."Antimiclobial Agents and Chemotherapy. 43(6). 1449-1458 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katayama, Y.Ito, T., K.Hiramatsu: "A new class of genetic element, staphylococcal cassette chromosome mec, encodes methicillin resistance in Staphlococcus aureus."Antimiclobial Agents and Chemotherapy. 44(6). 1549-1555 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C., and Hiramatsu, K.: "Complete structure of three types of staphylococcus cassette chromosome mec(SCCmec)ingegrated in the chromosome of MRSA strains in the world."Antimiclobial Agents and Chemotherapy. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤輝代: "Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin- resistant Staphylococcus aureus N315"Antimicrob,Agents and Chemother.. 43・6. 1449-1458 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 片山由紀: "A new class of genetic element, staphylococcal cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus"Antimicrob. Agents and Chemother.. 44・6. 1549-1555 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "Complete structure of three types of staphylococcus cassette chromosome mec (SCCmec) integrated in the chromosome of MRSA strains in the world."Antimicrob. Agents and Chemother.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "メチシリン耐性の伝播に関与するmobile genetic element (staphylo coccal cassette chromosome mec)"日本細菌学雑誌. 55・3. 483-498 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "S.aureusのvancomycinおよびmethicillin耐性"日本化学療法学会雑誌. 48・1. 7-23 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "疫学的解析と遺伝子検査-MRSAの分子疫学"臨床と細菌. 26. 177-182 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 片山由紀: "メチシリン耐性領域の正確な切り出しと部位特異的挿入"臨床と微生物. 26・2. 100 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "知っておきたい現代感染症事情"医歯薬出版株式会社. (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "臨床遺伝子医学ガイダンス"南山堂. 312 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "エマージングディジーズ"近代出版. 425 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "分子予防医学"医学書院. 384 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊藤輝代: "バイオサイエンスの新世紀 第13巻 生体防御"共立出版. 236 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Ito,Y.Katayama,and K.Hiramatsu: "Cloning and Seguence determindion of the entire mecDNA of pre-methicillin resistant Staphfococcus aureus N315"Antimicrobial Agents and Chemotherapy. 43(6). 1449-1458 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤輝代、片山由紀、平松啓一: "メチシリン耐性の伝幡に関与するmobile genetic element(SCCmec)"日本細菌学雑誌. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤輝代、花木英明、平松啓一: "S.aureusのvancomycinおよびmethicillin耐性"日本化学療法学会雑誌. 48(1). 7-23 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Hiramatsu,T.Ito,H.Hanaki: "Mechanism of methicillin and vancomycin resistance in staphylococcus aureus"Balliere's Clinical Infectious Diseases. 5(2). 221-242 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤輝代、平松啓一: "エマージングディジーズ"近代出版. 425 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤輝代、平松啓一: "しっておきたい現代感染症事情"医歯薬出版株式会社. 186 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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