| Project/Area Number |
11670281
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | University of Occupational and Environmental Health |
|---|
Principal Investigator |
TANIGUCHI Hatsumi University of Occupational and Environmental Health, School of Medicine, Professor, 医学部, 教授 (00037483)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | V.parahaemolyticus / a filamentous phage / a pandemic infection / serovar O3 : K6 / serovar O4 : K68 / Vf33-like / genetic transfer / virulence gene / 腸炎ビブリオ / 線維状ファージ / Vf33 / O3K6 / O4K68 / VfO3K6 / VfO4K68 / 遺伝子伝達 |
|---|
| Research Abstract |
We studied on a genetical analysis of a filamentous phage of V.parahaemolyticus in order to reveal a relation between the filamentous phage and the pandemic infection. Southern hybridization test showed that total DNAs isolated from serovar O3 : K6 and O4 : K68 strains harbored Vf33-like genome and the hybridization patterns were identical. We isolated Vf33-like filamentous phages from the strains, which were designated as VfO3 : K6 and VfO4 : K68. The genomes were integrated into a chromosomal DNA of a host cell. The genome sizes of VfO3 : K6 and VfO4 : K68 were 8784bp and 6891bp, respectively. VfO4 : K68 genome lacked 1893bp in a distinctive region of VfO3 : K6 genome. The genetic organization, nucleotide and amino acid sequences of genes encoding a replication, and virions' proteins were extremely homologous among Vf33, VfO3 : K6, and VfO4 : K68 genomes. Especially those of VfO3 : K6, and VfO4 : K68 were completely identical. However, a gene encoding an adsorption protein to a recep
… More
tor of a host cell was different between Vf33 and VfO3 : K6. This was consistent with a phenomenon that an indicator strain of Vf33 belonged a K38 serovar strain, and different from a O4 : K12 serovar strain of VfO3 : K6. On the other hand, a genetic structure of a region containing genes related to an integration/excision event and a regulation of replication was completely different among those of three phages. VfO3 : K6 possessed a novel gene on the distinctive region. VfO4 : K68 lacked the portion containing the gene, and acquired an another novel gene. These genes might be related to a pandemicity. This showed that the deletion was occurred on an excision event of a phage genome from a chromosomal state. Hybridization test indicated that almost non-pandemic strains also had homologous sequences with the filamentous phage genomes. The result showed the possibility that a filamentous phage widely existed among V.parahaemolyticus strains and played a important role in genetic transmission. Less
|
