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Function (s) of a novel Epstein-Barr virus latent gene, BARF0

Research Project

Project/Area Number 11670286
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionKochi Medical School

Principal Investigator

IMAI Shosuke  Kochi Medical School, Dpt of Microbiology, Professor, 医学部, 教授 (60232592)

Co-Investigator(Kenkyū-buntansha) KURODA Masayuki  Kochi Medical School, Dpt of Microbiology, Instructor, 医学部, 助手 (00253005)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsEpstein-Barr virus / BARF0 gene / Epithelial cells / Malignant change / 癌化
Research Abstract

Epstein-Barr virus (EBV) is closely associated with a B-cell tumor, Burkitt's lymphoma, and epithelial tumors, nasopharyngeal carcinoma and gastric carcinoma (GC). Among the known latent EBV genes, only a limited number of them, e.g. EBNA1, EBERs, LMP2A and recently identified BARF0, are expressed in the EBV-associated human malignancies. However, the function (s) of BARF0 is still unclear, whereas those of EBNA1, EBERs and LMP2A are being clarified. We investigated the possible role (s) of BARF0 in cellular malignant change by using the useful in vitro epithelial infection system by EBV, which we developed before.
Expression plasmids for EBNA1, EBERs, BARF0, RK-BARF0 and LMP2A were introduced independently into a cultured normal gastric epithelial cell, PGE-5 (J.Virol., 73 : 1286-92, 1999), and its cell clones expressing each gene were isolated by drug selection. Among those clones, BARF0- and RK-BARF0-expressing clones showed a significantly higher clonability in agar than EBNA1-, EBER-, LMP2A- and neo^r gene-transfected ones, though lower than EBV-infected counterparts. Another examination showed that, in many EBV-infected cell lines and biopsies from GC and NPC tissues, BARF0 was consistently expressed at the much higher level than RK-BARF0. Taken together, these results suggest that BARF0 has oncogenic potential, at least partially, for human gastric epithelial cells. Additionally, since the malignant phenotype seen in EBV-infected PGE-5 cells was not fully reproduced by BARF0 alone, other unidentified EBV latent gene (s) responsible for epithelial malignant change may exist.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Imai S, et al.: "Basic biology of Epstein-Barr virus and recent progress."Crit.Rev.Oncol.Hematol.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imai S, et al.: "Epstein-Barr virus infection of human epithelial cells."Curr.Topics Microbiol.Immunol.. 258(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzaki S, et al.: "Phylogeneticrelationship among T4-type bacteriophages in the Proteobacteria. In S.G.Pandalai(ed.)"Recent Research, Development in Virology. Transworld Research Network. Trivandrum India. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oda T, et al.: "Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and epithelial cells."Virology. 276. 52-58 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeda T, et al.: "Lytic Epstein-Barr virus infection in the synovial tissue of patients with rheumatoid arthritis."Arthritis Rheum.. 43(6). 1218-1225 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhang Y-W, et al.: "PEBP2αA/CBFA1 mutations in Japanese cleidocrania dysplasia patients."Gene. 244. 21-28 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzaki S, et al.: "Characterization of a novel Vibrio parahaemolyticus phage KVP241 and its relatives, frequently isolated from sea water."Microbiol.Immunol.. 44. 953-956 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imai S, et al.: "Basic biology of Epstein-Barr virus and recent progress."Crit.Rev.Oncol.Hematol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imai S, et al.: "Epstein-Barr virus infection of human epithelial cells."Curr.Topics Microbiol. Immunol.. 258 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzaki S, et al.: "Phylogeneticrelationship among T4-type bacteriophages in the Proteobacteria. In S.G.Pandalai (ed.)"Recent Research, Development in Virology. Transworld Research Network. Trivandrum India.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oda T, et al.: "Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and epithelial cells."Virology. 276. 52-58 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeda T, et al.: "Lytic Epstein-Barr virus infection in the synovial tissue of patients with rheumatoid arthritis."Arthritis Rheum.. 43(6). 1218-1225 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhang Y-W, et al.: "PEBP2 αA/CBFA1 mutations in Japanese cleidocrania dysplasia patients."Gene. 244. 21-28 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzaki S, et al.: "Characterization of a novel Vibrio parahaemolyticus phage KVP241 and its relatives, frequently isolated from sea water."Microbiol.Immunol.. 44. 953-956 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imai S, et al.: "Basic biology of Epstein-Barr virus and recent progress."Crit.Rev.Oncol.Hematol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imai S, et al.: "Epstein-Barr virus infection of human epithelial cells."Curr.Topics Microbiol.Immunol.. 258(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuzaki S, et al.: "Phylogeneticrelationship among T4-type bacteriophages in the Proteobacteria. In S.G.Pandalai (ed)."Recent Research, Development in Virology. Transworld Research Network. Trivandrum India. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Oda T, et al.: "Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and epithelial cells."Virology. 276. 52-58 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda T, et al.: "Lytic Epstein-Barr virus infection in the synovial tissue of patients with rheumatoid arthritis."Arthritis Rheum.. 43(6). 1218-1225 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhang Y-W, et al.: "PEBP2αA/CBFA1 mutations in Japanese cleidocrania dysplasia patients."Gene. 244. 21-28 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuzaki S, et al.: "Characterization of a novel Vibrio parahaemolyticus phage KVP241 and its relatives, frequently isolated from sea water."Microbiol.Immunol.. 44. 953-956 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeuchi R: "Respiratory syncytial virus infection of neonatal monocytes stimulates synthesis of in terferon regulatory factor-1 (IRF-1) and IL-1 βconverting enzyme (ICE)to secrete IL-1 β."J.Virol. 72(1). 837-840 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Imai S: "Cell-to-cell contact sa efficient mode of Epstein-Barr virus infection of diverse human epithelial cells."J.Virol. 72(5). 4371-4378 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsutsumi H: "A boy with fatal infectious mononucleosis suspected as first Japanese case of X-linked lymphoproliferative syndrome."Scand.J.Infect.Dis.. 30(6). 610-612 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Imai S: "virological basis of Epstein-Barr virus-positive gastric carcinoma."Gann Monograph on Cancer Res.. 45. 77-86 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishikawa J: "Epstein-Barr virus promotes epithelial cell growth in the absence of EBNA2 and LMP1 expression."J.Virol.. 73(2). 1286-1292 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugawara Y: "Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue : a novel EBV latency characterized by absence of EBV-encoded small RNA expression."Virology. 256(2). 196-202 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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