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Molecular mechanism of human herpesvirus 6 latent infection

Research Project

Project/Area Number 11670296
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

KONDO Kazuhiro  Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (70234929)

Co-Investigator(Kenkyū-buntansha) TAYA Keiko  Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (80263276)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordshuman herpesvirus 6 / latency / reactivation / macrophage / cytomegalovirus / Exanthem Subitum / ヒトヘルペスウイルス7 / 潜伏感染遺伝子
Research Abstract

We have studied the latent and the productive infection of HHV-6 in the peripheral blood macrophages. As for the study on HHV-6 latency, four kinds of latency-associated transcripts of human herpesvirus 6 were identified, which were detected only in latently infected cells. Although they were encoded in the same direction as the immediate early (IE) 1/2 genes and shared their protein-coding region with IE1/2, their transcription start sites and exon(s) were latency-associated. Our findings revealed that the structures, the encoded proteins, and the expression of the HHV-6 latency-associated transcripts were similar to those of HCMV latency-specific transcripts. The H6LTs and the HCMV latency-specific transcripts may have some common function during latency. The conserved features of the HHV-6 and HCMV latent transcripts that we observed in this study may assist us in understanding β-herpesvirus latency further. As for the study on the acute infection, we investigated the tropism of HHV-6 in peripheral blood mononuclear cells (PBMCs) during acute infection. We detected 637 ± 273 copies of viral DNA in 10^4 MO/Mφ. In contrast, in 10^4CD4^+T cells, which have been reported to be viral carriers during the acute infection of HHV-6, we found only 115 ± 42 copies of viral DNA. Consistent with these data, virus was isolated from MO/Mφ an order of magnitude more frequently than from CD4^+ T cells. Viral mRNA U79/80, which indicates viral replication, was detectable in the MO/Mφ. In addition, the mRNAs that encode viral chemokine receptors U12 and U51, which may modify the function of MO/Mφ, were expressed in the cells. Therefore, productively infected MO/Mφ may be the dominant cell population that is responsible for HHV-6 viremia during acute HHV-6 infection. The strong interaction of HHV-6 with MO/Mφ may be partly responsible for the pathogenesis of this virus.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] K.Kondo et al.: "Identification of human Herpesvirus 6 latency-associated transcripts"J. Virol. 76(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Kondo et al.: "Strong interaction between human Herpesvirus 6 and peripheral blood monocytes/macrophages during acute infection"J Med Virol. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hirata et al.: "Human herpesvirus 6 down-regulates major histocompatibility complex class I in dendritic cells"J Med Virol. 65. 576-583 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Tanaka-Taya et al.: "Reactivation of human herpesvirus 6 by infection of human herpesvirus 7"J Med Virol. 60. 284-289 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Taniguchi et al.: "Structure of transcripts and proteins encoded by U79-80 of human herpesvirus 6 and its subcellular localization in infected cells"Virology. 271. 307-320 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kondo, K. Shimada, J. Sashihara, K. et al.: "Identification of human Herpesvirus 6 latency- associated transcripts"J. Virol.. 76 (8)(in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kondo, T. Kondo, K. Shimada et al.: "Strong interaction between human herpesvirus 6 and peripheral blood monocytes/macrophages during acute infection"J. Med. Virol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Hirata, K. Kondo and K. Yamanishi: "Human herpesvirus 6 down-regulates major histocompatibility complex class I in dendritic cells"J. Med. Virol.. 65. 576-583 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Tanaka-Taya, K. Kondo, K. Yamanishi et al.: "Reactivation of human herpesvirus 6 by infection of human herpesvirus 7"J. Med. Virol.. 60 (3). 284-289 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Taniguchi, K. Kondo, K. Yamanishi et al.: "Structure of transcripts and proteins encoded by U79-80 of human herpesvirus 6 and its subcellular localization in infected cells"Virology. 271 (2). 307-320 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi T. et al.: "Structure of transcripts and proteins encoded by U79-80 of human herpesvirus 6 and its subcellular localization in infected cells."Virology.. 271・2. 307-320 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka-Taya K et al.: "Reactivation of human herpesvirus 6 by infection of human herpesvirus 7."Journal of Medical Virology. 60・3. 284-289 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taniguchi T.et al.: "Structure of transcripts and proteins encoded by U79/80 of HHV-6 and its subcellular localization in infected cells"Viology. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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