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Studies on apoptosis induced in the virus-infected cells

Research Project

Project/Area Number 11670298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionThe University of Tokushima

Principal Investigator

KOYAMA Hajime  The University of Tokushima, School of Medicine, Associate Professor, 医学部, 助教授 (80109074)

Co-Investigator(Kenkyū-buntansha) ADACHI Akio  The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (90127043)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsapoptosis / antiapoptosis / sorbitol / virus / phagocytosis / defense mechanism / 抗ウイルス作用
Research Abstract

To understand the biological significance of virus-induced apoptosis in animal virus infection, we quantitatively characterized apoptosis and virus multiplication in the virus-infected cells. Based on the observation that the multiplication of the insect virus was abolished when the infected cells induced apoptosis, virus-induced apoptosis has been considered to be one of the host defense mechanism against virus invasion. However, in contrast to the infection with insect viruses, influenza virus and some RNA animal viruses can grow rapidly under the conditions that the infected cells induced apoptosis. The progeny virus yield reached the maximum level at the time when these infected cells induced apoptosis, indicating influenza virus is able to overcome apoptosis of the infected cells by a rapid multiplication.
On the other hand, we found that HEp-2 cells induced massive apoptosis immediately after the treatment with sorbitol, one of the polyhydric alcohol. By the use of sorbitol-treatment, we showed that (1) animal viruses are able to grow in the apoptotic cells, (2) large DNA viruses, such as herpes simplex virus type one (HSV-1) and type 2 (HSV-2), overcome apoptosis by multiple antiapoptosis genes of the viruses which allow the viruses to suppress the induction of apoptosis in the infected cells, and (3) one of antiapoptosis genes of HSV-2 is US3 gene.
These results indicate that the biological significance of apoptosis in the animal virus infection is not the abortion of virus multiplication by premature death of the infected cells and suggest that apoptosis works as a host defense mechanism by converting the virus-infected cells to the target of phagocytotic activity of macrophages in the infected animal.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hata S.: "Antiapoptotic activity of herpes simplex virus type 2 : A role of US3 protein kinase gene."Microbes Infect.. 1. 601-607 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kurokawa M.: "Influenza virus overcomes appotosis by rapid multiplication."Int.J.Mol.Med.. 3. 527-530 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyama A.H.: "Characterization of apoptosis induced by sorbitol : a unique system for the detection of antiapoptotic activities of viruses."Microbes Infect.. 2. 599-606 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fukumori T.: "Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr."Microbes Infect.. 2. 1011-1017 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyama A.H.: "Physiological significance of apoptosis in animal virus infection."Microbes Infect.. 2. 1111-1117 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hata S., Koyama A.H., Shiota H., Adachi A., Goshima F., Nishiyama Y.: "Antiapoptotic activity of herpes simplex virus type 2 : A role of US3 protein kinase gene."Microbes Infect.. 1. 601-607 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kurokawa M., Koyama A.H., Yasuoka S., Adachi A.: "Influenza virus overcomes appotosis by rapid multiplication."Int.J.Mol.Med.. 3. 527-530 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyama A.H., Arakawa T., Adachi A.: "Characterization of apoptosis induced by sorbitol : a unique system for the detection of antiapoptotic activities of viruses."Microbes Infect.. 2. 599-606 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fukumori T., Akari H., Yoshida A., Fujita M., Koyama A.H., Adachi A.: "Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr."Microbes Infect.. 2. 1011-1017 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyama A.H., Fukumori T., Fujita M., Irie H., Adachi A.: "Physiological significance of apoptosis in animal virus infection."Microbes Infect.. 2. 1111-1117 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyama A.H.: "Characterization of apoptosis induced by sorbitol : a unique system for the detection of antiapoptotic activities of viruses."Microbes Infect.. 2. 599-606 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukumori T.: "Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr."Microbes Infect.. 2. 1011-1017 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koyama A.H.: "Physiological significance of apoptosis in animal virus infection."Microbes Infect.. 2. 1111-1117 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koyama,A.H.: "Comparison of an antiviral activity of recombinant consensus interferon with・・・・."Microbes and Infection. 1. 1073-1077 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hata,S.: "Antiapoptotic activity of herpes simplex virus type 2:the role of US3 protein・・・・."Microbes and Infection. 1. 601-607 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kurokawa,M.: "Influenza virus overcomes apoptosis by rapid multiplication."International Journal of Molecular Medicine. 3. 527-530 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Adachi,A.: "Activation of HIV-1 enhancer sequence by vaccinia virus."International Journal of Molecular Medicine. 3. 311-313 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iida,S.: "Compatibility of Vpu-like activity in the four groups of primate immunodeficiency・・・."Virus Genes. 18. 183-187 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akira,H.: "Pseudotyping HIV-1 by vesicular stmatitis virus G protein does not reduce the・・・・."Journal of General Virology. 80. 2945-2949 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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