| Project/Area Number |
11670301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | University of Shizuoka |
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Principal Investigator |
SUZUKI Takashi University of Shizuoka, School of Pharmaceutical Science, Associate Professor, 薬学部, 助教授 (20240947)
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| Co-Investigator(Kenkyū-buntansha) |
HIDARI Kazuya University of Shizuoka, School of Pharmaceutical Science, Research Associate, 薬学部, 助手 (20260226)
SUZUKI Yasuo University of Shizuoka, School of Pharmaceutical Science, Professor, 薬学部, 教授 (00046278)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | influenza Virus / Sialic acid / Host range / sulfatide / hemagglutinin / N-acetylnevramic acid / N-glycolylneramic acid |
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| Research Abstract |
This project aims at explicating the role of hemagglutinin on the replication of influenza viruses and transmission of the viruses between the hosts.
The results of the project are as follows.
1. We tested the binding reactivity of human H3 influenza A viruses, which were isolated from 1968 to 1979, to sialylglycolipids containing different molecular species of terminal sialic acids. All human H3 viruses tested bound both Neu5Ac and Neu5Gc.
2. We examined the relative abundance of Neu5Ac and Neu5Gc in the epithelial cells of horse trachea. More than 90 % of the sialic acid was Neu5Gc, while the remaining proportion was NeuAc. The major sialic acid-Galactose moiety present in horse tracheal epithelial cells is Neu5Gcα2.3Gal. We examined the requirement for the hemagglutinin for support of viral replication in horses, using viruses whose Has differ in receptor specificity. The results indicated that recognition of Neu5Gcα2,3Gal moiety is critical for viral replication in horses.
3. The recognition of a glycoconjugate moiety possessing Neu5Gcα2,3Gal was associated with viral replication in duck intestine.
4. Sulfatide binding site is localized in the sialic acid binding pocket of HA or the neighborhood of the pocket.
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