| Project/Area Number |
11670322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Hiroshima University |
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Principal Investigator |
FURUSAWA Shuichi Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (80130037)
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| Co-Investigator(Kenkyū-buntansha) |
HORIUCHI Hiroyuki Hiroshima University, Faculty of Applied Biological Science, Research Associate, 生物生産学部, 助手 (80243608)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Germinal center / T cells / Receptor Editting / Internal heptemer / TCR / Repertoire / Chicken |
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| Research Abstract |
To evaluate the chicken folliclular immunen system, GC formation and the role of T cells are studied. A single GC was isolated from the chikcen spleen, immunized 7 days or 14 days earlier with antigen by the intravenous route. Rearranged TCR genes were amplified using primers for Vβ1 and Jβ. Sequence analysis revealed only few patterns of CDR3. The number of patern in 7 days-GC were 7/19, 4/16, 12/22, 7/10 (mean 10.3±1.4 patern/22 clone ; 46. 8%). The number of patern in 14 days-GC was 2/39 (5.1%). 1) These findings indicate that there is restricted T cell clonality within a single GC following antigen stimulation, and that the focusing of T cell repertory was took placen during GC formation. 2) RAG mRNA expression in GC was also observed at 5 days-GC.3) Deletion circular DNA, pruduced at V-D recmobination was observed in thymus, spleen, Peyer's patch, difusely infiltrated area of BF and 8 days-GC.
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