| Project/Area Number |
11670342
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Kobe University |
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Principal Investigator |
LEE Myeong jin Kobe University School of Medicine Public Health, Research Associate, 医学部, 助手 (20273766)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIO Hisahide Kobe University School of Medicine Public Health, Associate Professor, 医学部, 助教授 (80189258)
SUMINO Kimiaki Kobe University School of Medicine Public Health, Professor, 医学部, 教授 (90030832)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | endocrine disruptor / Microphysiometer System / MCF-7 cell / β-estradiol / estrogen receptor α / cadmium / 内分泌撹乱物質 / M1WT3細胞 / carbachol |
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| Research Abstract |
We applied a microphysiometer, Cytosensor^<TM> Microphysiometer System to evaluate the effects of endocrine disruptors on the cell metabolisms. The Cytosensor^<TM> monitors the metabolic changes affected by receptor-ligand binding as changes in the rate of acidification of the medium surrounding the cells. Recently, cadmium has been considered as a candidate endocrine disruptor. In order to clarify the effects of cadmium on estrogen receptor, we examined cellular response of MCF-7 cells with the hormonal receptor protein using Cytosensor^<TM>. Exposure of 10^<-5> M β-estradiol increased acidification rate of the medium surrounding the cells. However, when treated with 10^<-4> M CdCl_2 or combination of 10^<-4> M CdCl_2 and 10^<-5> M β-estradiol, acidification rate of MCF-7 cells was decreased. The CdCl_2 at the concentration of less than 10^<-5> M did not affect acidification rate. 10^<-4> M CdCl_2 also decreased acidification rate of CHO-KIM cells which expressed muscarine receptor. These results indicated that highly concentrated Cd did not have selective inhibition of metabolism via estrogen receptor a or muscarine receptor and that it showed other cytotoxic effects involving the whole metabolism in the cells.
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