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Involvement of UDP-GlcNAc biosynthesis in sexual dysfunctions caused by endocrine disrupting chemicals

Research Project

Project/Area Number 11670348
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionYokohama City University

Principal Investigator

OKABE Toshiko  Yokohama City University, School of Medicine, Lecturer, 医学部, 講師 (20152564)

Co-Investigator(Kenkyū-buntansha) MATSUI Mitsuaki  Yokohama City University, School of Medicine, Research Assciate, 医学部, 助手 (00285115)
KASHIMA Yuji  Yokohama City University, School of Medicine, Research Associate, 医学部, 助手 (50233705)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmale infertility / UDP-Ν-acetylglucosamine / UAP1 / AgX1 / anti-sperm antibody / GlcNAc kinase / GlcNAc-phosphate deacetyiase / NAG cluster / N-アセチルグルコサミン / 精子運動率 / AgX-1 / Agx-1 / 内分泌かく乱物質
Research Abstract

In human and animals, male sexual dysfunctions had been reported recently. It is suggested that the reason for this phenomenon was thought to be the effects of endocrine disrupting chemicals on sex hormone receptors. Human N-acetylglucosamine-1-phosphate uridyltransferase (UAP1) is expressed in testis and sperm tail, especially outer dense fiber and thought to play important function in sexual function. In this study, we found that the antibody against UAP1 was detected more frequently in sperm plasma with impaired sperm motility. Because the primary structure of UAP1 showed the partial homology with several sperm surface proteins, the antibody against UAP1 might prevent the function of UAP1 as well as other sperm surface proteins, and cause the inhibition of various sperm function.
Furthermore, GNA1 and AGM1, which encode glucosamine-6-phosphate acetyltransferase and phosphoacetylglucosamine mutase, respectively, and are involved in eukaryotic UDP-GlcNAc biosynthesis as well as UAP1 had been cloned and characterized. Furthermore the enzymes for catabolism of N-acetylglucosamine (GlcNAc) such as GlcNAc kinase and GlcNAc-phosphate deacetylase were identified and characterized.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Mio T.et al.: "Saccharomyces cerevisiae GNA I, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylgluocosamine synthesis"J.Biol.Chem. 274. 424-429 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mio T.et al.: "Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase"Biochim Biophys Acta. 1492. 369-376 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada-Okabe T. et al.: "Identification and characterization of the gene for N-acetylglucosamine kinase and N-acetylglucosamine-phosphate deacetylase in the pathogenic fungus Candida albicans"Eur.J.Biochem. 268. 2498-2505 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada-Okabe T., Yamada-Okabe H.: "Characterization of the CaNAG3, CaNAG4 and CaNAG6 genes of the pathogenic fungus Candida albicans"(submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mio T, Yamada-Okabe T and Yamada-Okabe H: "Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-Ν-acetylgluocosamine synthesis"J Biol Chem. 274. 424-429 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mio T, Yamada-Okabe T, Arisawa M and Yamada-Okabe H: "Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase"Biochim Biophys Acta.. 1492. 369-376 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada-Okabe T, Sakamori Y, Mio T and Yamada-Okabe H: "Identification and characterization of the gene for Ν-acetylglucosamine kinase and Ν-acetylglucosamine-phosphate deacetylase in the pathogenic fungus Candida albicans"Eur J Biochem. 268. 2498-2505 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada-Okabe T and Yamada-Okabe H: "Characterization of the CaNAG3, CaNAG4 and CaNAG6 genes of the pathogenic fungus Candida albicans"(submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada-Okabe T et al.: "Identification and characterization of the genes for N-acetylglucosamine kinase and N-acetylglucosamine-phosphate deacetylase in the pashogenic fungus Candida albicans"European Journal of Biochemistry. 268. 2498-2505 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada-Okabe T et al: "Identification and characterization of the genes for N-acetylglucosamine kinase and N-acetyglucosamine-phosphate deacetylase in the pathogenic fungus Canadida alhicans."European Journal of Biochemistry. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ono.N et al: "The yeast Chs4 protein stimulates the trypsin-sensitive activity of chitin synthase 3 through an apparent protein-protein interaction."Microbiology. 146. 385-391 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mio T et al: "Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase"Biochimica et Biophysica Acta. 1492. 369-376 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mio, T et al: "Saccharomyces cereviaise GNA1, an essential gene encoding a novel acetyltransferse invoved in UDP-N-acetylglucosamine synthesis"Journal of Biological Chemistry. 274(1). 424-429 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada-Okabe,T et al: "Roles of three histidine kinase genes in hyphal development and virulence of the pathogenic funfus Candida albicans"Journal of Bacteriology. 181(23). 7243-7247 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada-Okabe, T et al: "The Candida albicans gene for mRNA 5'-cap methyltransferase: identification of additional residues essentail for catalysis"Microbiology. 145. 3023-3033 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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