| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Research Abstract |
The trichloroacetic acid (TCA) is the chemicals mainly used with the purpose of the deproteinization in medicine and genetic engineering. Moreover, it is used as herbicide and antiseptics. There are some reports of synthesizing as a by-product in the disinfection, In the meantime, there is the toxicity of the liver function on the TCA in rats and mice. In this study, the movement of TCA was examined in vitro and in vivo for the purpose of the appropriate evaluation of the bio-monitoring.
In vitro, 38.5mg/kg of trichloroethylene (TRI) and 39.2mg/kg of tetrachloroethylene (PCE) were added to the homogenate of the mid-gut gland of clams. These concentrations tended to decrease a little after 3 and 6h. However, the differences were not significant. In both of TRI and PCE, TCA and trichloroethanol (TCE) were under the quantitive lower limit value in the sample after 3h incubation. The TCA was detected in the trace in the sample after 6h incubation, and the TCE was under the quantitive lower limit value, From these results, it was indicated that TRI and PCE were metabolized in the mid-gut gland of clam in the TCA.
In vivo, (1) : the concentration of TCA in clam incubated in the 10,1 and 0.1mg/l of TCA water solutions during 24h at 10℃ was 4.8, 0.78 and 0.09mg/kg, respectively. It was recognized to accumulate. Especially, the tendency was remarkable in case of the low concentration. (2) : The distribution of the TCA at four parts of flesh tended to be similar as case of (1) at each concentration. The concentration of the TCA was high in order of water tube, mid-gut gland, mantle and foot. It was guessed that TCA was metabolized to the dichloroacetic acid (DCA) in clams, because the TCA was distributed in the mid-gut gland.
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