Mechanism of glucose intolerance induced by a low-carbohydrate diet
| Project/Area Number |
11670365
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | 山梨医科大学 |
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Principal Investigator |
KANEKO Takashi Yamanashi Medical University, Department of Environmental Health, Associate Professor, 医学部, 助教授 (10233876)
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| Co-Investigator(Kenkyū-buntansha) |
WANG Pei-yu Yamanashi Medical University, Department of Environmental Health, Assistant Professor, 医学部, 助手 (10283201)
MAEDA Shuichiro Yamanashi Medical University, First Department of Biochemistry, Professor, 医学部, 教授 (10117244)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Type2diabetesmellitus / Carbohydrate / Glucose-fattyacidcycle / Rat / Glucose tolerance / Insulin secretion / Insulin sensitivity |
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| Research Abstract |
In this study, we clarified the mechanism of glucose intolerance induced by a low-carbohydrate (LC) diet. We examined how long a LC diet must be consumed in order to impair glucose tolerance in rats, and whether insulin secretion and insulin sensitivity are affected by the increased level of free fatty acids cFEA) induced by the diet. Intraperitoneal glucose tolerance tests were performed onmale Wistar rats that were fed a control diet (carbohydrate 6050 or aLC (carbohydrate 1050 diet for 1, 3, or 7 day(s) in a randomized crossover desiga Insulin release was measured in pancreatic islets isolated from rats fed control and LC diets for 3 days. Insulin secretion was also examined in islets exposed to palmitic acid (PA) during 244i culture. Whole body insulin sensitivity was measured by an intravenous insulin tolerance test in rats fed LC diet for 3 days or in rats intravenously in fused withPA for 24 h. Glucose tolerancewas impaired after 3 and 7 days of consuming a LC diet ; the impairment was more severe after 7 days. The glucose tolerance impairment was accompanied by a rise in the fasting plasma FFA level. Glucose-stimulated insulin secretion was significantly reduced in pancreatic islets from rats fed LC diet for 3 days. Exposure of control rat islets to PA impaired glucose-stimulated insulin secretion Rats fed LC diet for 3 days developed insulin resistance inviva Intravenous PA infusion into rats decreased thewhole body insulin sensitivity. Feeding rats a LC diet for 3 days impaired glucose tolerance The impairment may be induced by a decrease in both insulin secretion and insulin sensitivity, \rtiich may be caused by the increased plasma FFA level due to the LC diet.
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Report
(4 results)
Research Products
(12 results)
