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A novel method for detecting either the maternal or paternal allele by using the DNA methylation difference between parents in the imprinted region

Research Project

Project/Area Number 11670409
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionNiigata University

Principal Investigator

NAITO Emiko  Niigata Univ., School of Medicine Assistant, 医学部, 助手 (80018811)

Co-Investigator(Kenkyū-buntansha) DEWA Koji  Niigata Univ., School of Medicine Associate Professor, 医学部, 助教授 (10197832)
YAMANOUCHI Haruo  Niigata Univ., School of Medicine Professor, 医学部, 教授 (30134919)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordspaternity testing / PCR method / repeat polymorphism / methylation / imprinting / H19gene / リピート配列 / 多型
Research Abstract

In mammals, imprinted genes are preferentially expressed from either the maternal or paternal allele. Several recent observations show that DNA methylation plays an important role in the imprinted inheritance of the gene. Generally, An individual inherits two alleles together from his parents and the origin of each allele can be determined by typing the parents. The aim of this study is to detect a paternally or maternally derived allele from one person by using the methylation difference in the imprinted region. For the purpose, useful probe within the region is requisite as the analytic target.
In this study, forensic utility of the VNTR locus which is located approximately 7.6kb upstream of the H19 gene that is maternally expressed was evaluated. The human 5'H19 flanking sequence was searched from DDBJ.Allele frequency of the VNTR locus was examined in 200 unrelated Japanese. Seven alleles and 22 genotypes were identified. Its heterozygosity and polymorphism information content were 0.749 and 0.669, respectively. The distribution has fitted Hardy-Weinberg equilibrium(p>0.100). In a case study, this genetic typing was successfully applicated to the personal identification of a decomposed forensic sample. These results indicated that the VNTR in the H19 5'flank showed to be highly polymorphic and useful for identification of Japanese individuals. This suggests the polymorphic repeat has become useful probe in the aimed method.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Emiko Naito: "A Novel dimorphism in the human SRY gene : usefulness in human migration studies"International Journal of Legal Medicine. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Gotaro Watanabe: "A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers"Electrophoresis. 22. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isao Yuasa: "Distribution of a C-to-T polymorphism in the Sex-Determining Region Y(SRY) Gene"Progress in Forensic Genetics 8. 321-323 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshiharu Honma: "Identification by histological and microsatellite analyses of a stranded Beaked Whale as that struck previously by a jetfoil operating in the sea of Japan"Fisheries Science. 65. 547-552 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Emiko Natio, Kazuo Umetsu, Isao Yuasa, Koji Dewa, Hirokazu Sumi, Haruo Ymanouchi: "A novel dimorphism in the human SRY gene : usefulness in human migration studies."Interanational Journal of Legal Medicine. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Gotaro Watanabe, Kazuo Umetsu, Isao Yuasa, Michihiko Sato, Munechika Sakabe, Emiko Naito, Haruo Yamanouchi, Tsuneo Suzuki: "A novel technique for detecting single nucleotide polymorphism by analyzing consumed allele-specific primers."Electrophoresis. 22. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Yuasa, K.Umetsu, E.Naito, A.Miyoshi, K.Ago and Y.Irizawa: "Distribution of a C-to-T Polymorphism in the Sex-Determining Region Y(SRY)Gene."Genetics. 8. 321-323 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honma Y., Ushiki T., Takeda M., Naito E., Dewa K and Yamanouchi H.: "Identification by Histological and Microsatellite Analyses of a Stranded Beaked Whale as that Struck Previously by a Jetfoil Operating in the Sea of Japan."Fisheries Science. 65. 547-552 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Emiko Naito: "A novel dimorphism in the human SRY gene usefulness in human migration studies"International Journal of Legal Medicine. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Gotaro Watanabe: "A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers"Electrophoresis. 22. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Isao Yuasa: "Distribution of a C-to-T Polymorphism in the Sex-Determining Region Y (SRY) Gene"Progress in Forensic Genetics 8. 321-323 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 内藤笑美子: "Y染色体上の3種類のbiallelic markerのハプロタイプ分析"DNA多型. 8. 165-166 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshiharu Honma: "Identification by histological and microsatellite analyses of a stranded Beaked whale as that struck previously by a jetfoil operating in the Sea of Japan"Fisheries Science. 65. 547-552 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshiharu Honma: "Identification by histological and microsatellite analyses of a stranded Beaked Whale as that struck previously by a jetfoil operating in the Sea of Japan"Fisheries Science. 65. 547-552 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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